Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility

Sarisoy, Alp; Acosta, Sergio; Rodríguez-Cabello, José Carlos; Czichowski, Phillip; Kopp, Alexander; Jockenhoevel, Stefan; Fernández-Colino, Alicia

doi:10.3390/ijms24076776

Open AccessArticle

Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility

by

Alp Sarisoy

^1,†

,

Sergio Acosta

^1,†

,

José Carlos Rodríguez-Cabello

²,

Phillip Czichowski

³,

Alexander Kopp

³

,

Stefan Jockenhoevel

^1,4,*

and

Alicia Fernández-Colino

^1,*

¹

Department of Biohybrid & Medical Textiles (BioTex), AME–Institute of Applied Medical Engineering, Helmholtz Institute, RWTH Aachen University, D-52074 Aachen, Germany

²

Bioforge Lab, Group for Advanced Materials and Nanobiotechnology, Biomedical Networking Research Center of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Edificio LUCIA, Universidad de Valladolid, 47011 Valladolid, Spain

³

Fibrothelium GmbH, D-52068 Aachen, Germany

⁴

AMIBM-Aachen-Maastricht-Institute for Biobased Materials, Faculty of Science and Engineering, Brightlands Chemelot Campus, Maastricht University, 6167 RD Geleen, The Netherlands

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2023, 24(7), 6776; https://doi.org/10.3390/ijms24076776

Submission received: 23 February 2023 / Revised: 24 March 2023 / Accepted: 3 April 2023 / Published: 5 April 2023

(This article belongs to the Collection Feature Papers in Materials Science)

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Abstract

More than 260 million surgical procedures are performed worldwide each year. Although sutures and staples are widely used to reconnect tissues, they can cause further damage and increase the risk of infection. Bioadhesives have been proposed as an alternative to reconnect tissues. However, clinical adhesives that combine strong adhesion with cytocompatibility have yet to be developed. In this study, we explored the production of adhesives based on protein-engineered polymers bioinspired by the sequence of elastin (i.e., elastin-like recombinamers, ELRs). We hypothesized that the combination of polyphenols (i.e., tannic acid, TA) and ELRs would produce an adhesive coacervate (ELR+TA), as reported for other protein polymers such as silk fibroin (SF). Notably, the adhesion of ELR alone surpassed that of ELR+TA. Indeed, ELR alone achieved adhesive strengths of 88.8 ± 33.2 kPa and 17.0 ± 2.0 kPa on porcine bone and skin tissues, respectively. This surprising result led us to explore a multicomponent bioadhesive to encompass the complementary roles of elastin (mimicked here by ELR) and silk fibroin (SF), and subsequently mirror more closely the multicomponent nature of the extracellular matrix. Tensile testing showed that ELR+SF achieved an adhesive strength of 123.3 ± 60.2 kPa on porcine bone and excellent cytocompatibility. To express this in a more visual and intuitive way, a small surface of only 2.5 cm² was able to lift at least 2 kg of weight. This opens the door for further studies focusing on the ability of protein-engineered polymers to adhere to biological tissues without further chemical modification for applications in tissue engineering.

Keywords: tissue adhesive; protein polymer; elastin-like recombinamer; tannic acid; silk fibroin; bone; skin

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MDPI and ACS Style

Sarisoy, A.; Acosta, S.; Rodríguez-Cabello, J.C.; Czichowski, P.; Kopp, A.; Jockenhoevel, S.; Fernández-Colino, A. Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility. Int. J. Mol. Sci. 2023, 24, 6776. https://doi.org/10.3390/ijms24076776

AMA Style

Sarisoy A, Acosta S, Rodríguez-Cabello JC, Czichowski P, Kopp A, Jockenhoevel S, Fernández-Colino A. Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility. International Journal of Molecular Sciences. 2023; 24(7):6776. https://doi.org/10.3390/ijms24076776

Chicago/Turabian Style

Sarisoy, Alp, Sergio Acosta, José Carlos Rodríguez-Cabello, Phillip Czichowski, Alexander Kopp, Stefan Jockenhoevel, and Alicia Fernández-Colino. 2023. "Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility" International Journal of Molecular Sciences 24, no. 7: 6776. https://doi.org/10.3390/ijms24076776

APA Style

Sarisoy, A., Acosta, S., Rodríguez-Cabello, J. C., Czichowski, P., Kopp, A., Jockenhoevel, S., & Fernández-Colino, A. (2023). Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility. International Journal of Molecular Sciences, 24(7), 6776. https://doi.org/10.3390/ijms24076776

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Bioglues Based on an Elastin-Like Recombinamer: Effect of Tannic Acid as an Additive on Tissue Adhesion and Cytocompatibility

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