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Article

Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration

by
Annamaria Tisi
1,
Giulia Carozza
1,
Alessandro Leuti
2,3,
Rita Maccarone
1,* and
Mauro Maccarrone
1,3,*
1
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
2
Department of Medicine, Campus Bio-Medico University of Rome, 00128 Rome, Italy
3
European Center for Brain Research (CERC)/Santa Lucia Foundation IRCCS, 00143 Rome, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(7), 6749; https://doi.org/10.3390/ijms24076749
Submission received: 22 February 2023 / Revised: 31 March 2023 / Accepted: 3 April 2023 / Published: 4 April 2023
(This article belongs to the Special Issue Retinal Degenerative Diseases)
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Abstract

Resolvin E1 (RvE1) is an eicosapentaenoic acid-derived lipid mediator involved in the resolution of inflammation. Here, we investigated whether RvE1 alterations may occur in an animal model of age-related macular degeneration (AMD). To this end, Sprague Dawley albino rats underwent light damage (LD), and retinas and serum were analyzed immediately or seven days after treatment. Western blot of retinas showed that the RvE1 receptor ChemR23 and the RvE1 metabolic enzymes 5-LOX and COX-2 were unchanged immediately after LD, but they were significantly up-regulated seven days later. Instead, the RvE1 receptor BLT1 was not modulated by LD, and neither was the RvE1 degradative enzyme 15-PGDH. Moreover, ChemR23, 5-LOX, COX-2 and BLT1 were found to be more expressed in the inner retina under all experimental conditions, as observed through ImageJ plot profile analysis. Of note, amacrine cells highly expressed BLT1, while ChemR23 was highly expressed in the activated microglia of the outer retina. ELISA assays also showed that LD rats displayed significantly higher circulating levels and reduced retinal levels of RvE1 compared to controls. Altogether, our data indicate that RvE1 metabolism and signaling are modulated in the LD model, suggesting a potentially relevant role of this pathway in AMD.
Keywords: bioactive lipids; SPMs; RvE1; age-related macular degeneration; light damage bioactive lipids; SPMs; RvE1; age-related macular degeneration; light damage

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MDPI and ACS Style

Tisi, A.; Carozza, G.; Leuti, A.; Maccarone, R.; Maccarrone, M. Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration. Int. J. Mol. Sci. 2023, 24, 6749. https://doi.org/10.3390/ijms24076749

AMA Style

Tisi A, Carozza G, Leuti A, Maccarone R, Maccarrone M. Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration. International Journal of Molecular Sciences. 2023; 24(7):6749. https://doi.org/10.3390/ijms24076749

Chicago/Turabian Style

Tisi, Annamaria, Giulia Carozza, Alessandro Leuti, Rita Maccarone, and Mauro Maccarrone. 2023. "Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration" International Journal of Molecular Sciences 24, no. 7: 6749. https://doi.org/10.3390/ijms24076749

APA Style

Tisi, A., Carozza, G., Leuti, A., Maccarone, R., & Maccarrone, M. (2023). Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration. International Journal of Molecular Sciences, 24(7), 6749. https://doi.org/10.3390/ijms24076749

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