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Article

The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes

by
Ilona Uzieliene
1,
Daiva Bironaite
1,
Rokas Miksiunas
1,
Edvardas Bagdonas
1,
Raminta Vaiciuleviciute
1,
Ali Mobasheri
1,2,3,4 and
Eiva Bernotiene
1,*
1
Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, 08406 Vilnius, Lithuania
2
Research Unit of Health Sciences and Technology, Faculty of Medicine, University of Oulu, 90014 Oulu, Finland
3
World Health Organization Collaborating Center for Public Health Aspects of Musculoskeletal Health and Aging, Université de Liège, B-4000 Liège, Belgium
4
Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(7), 6730; https://doi.org/10.3390/ijms24076730
Submission received: 13 January 2023 / Revised: 27 February 2023 / Accepted: 29 March 2023 / Published: 4 April 2023
(This article belongs to the Special Issue Ion Channels as Therapeutic Target: Drug Design and Pharmacological Investigation)
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Abstract

Cartilage is an avascular tissue and sensitive to mechanical trauma and/or age-related degenerative processes leading to the development of osteoarthritis (OA). Therefore, it is important to investigate the mesenchymal cell-based chondrogenic regenerating mechanisms and possible their regulation. The aim of this study was to investigate the role of intracellular calcium (iCa2+) and its regulation through voltage-operated calcium channels (VOCC) on chondrogenic differentiation of mesenchymal stem/stromal cells derived from human bone marrow (BMMSCs) and menstrual blood (MenSCs) in comparison to OA chondrocytes. The level of iCa2+ was highest in chondrocytes, whereas iCa2+ store capacity was biggest in MenSCs and they proliferated better as compared to other cells. The level of CaV1.2 channels was also highest in OA chondrocytes than in other cells. CaV1.2 antagonist nifedipine slightly suppressed iCa2+, Cav1.2 and the proliferation of all cells and affected iCa2+ stores, particularly in BMMSCs. The expression of the CaV1.2 gene during 21 days of chondrogenic differentiation was highest in MenSCs, showing the weakest chondrogenic differentiation, which was stimulated by the nifedipine. The best chondrogenic differentiation potential showed BMMSCs (SOX9 and COL2A1 expression); however, purposeful iCa2+ and VOCC regulation by blockers can stimulate a chondrogenic response at least in MenSCs.
Keywords: intracellular calcium ions; voltage-operated calcium channels (VOCC); mesenchymal stem/stromal cells (MSC); chondrocytes; chondrogenic differentiation intracellular calcium ions; voltage-operated calcium channels (VOCC); mesenchymal stem/stromal cells (MSC); chondrocytes; chondrogenic differentiation

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MDPI and ACS Style

Uzieliene, I.; Bironaite, D.; Miksiunas, R.; Bagdonas, E.; Vaiciuleviciute, R.; Mobasheri, A.; Bernotiene, E. The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes. Int. J. Mol. Sci. 2023, 24, 6730. https://doi.org/10.3390/ijms24076730

AMA Style

Uzieliene I, Bironaite D, Miksiunas R, Bagdonas E, Vaiciuleviciute R, Mobasheri A, Bernotiene E. The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes. International Journal of Molecular Sciences. 2023; 24(7):6730. https://doi.org/10.3390/ijms24076730

Chicago/Turabian Style

Uzieliene, Ilona, Daiva Bironaite, Rokas Miksiunas, Edvardas Bagdonas, Raminta Vaiciuleviciute, Ali Mobasheri, and Eiva Bernotiene. 2023. "The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes" International Journal of Molecular Sciences 24, no. 7: 6730. https://doi.org/10.3390/ijms24076730

APA Style

Uzieliene, I., Bironaite, D., Miksiunas, R., Bagdonas, E., Vaiciuleviciute, R., Mobasheri, A., & Bernotiene, E. (2023). The Effect of CaV1.2 Inhibitor Nifedipine on Chondrogenic Differentiation of Human Bone Marrow or Menstrual Blood-Derived Mesenchymal Stem Cells and Chondrocytes. International Journal of Molecular Sciences, 24(7), 6730. https://doi.org/10.3390/ijms24076730

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