Hydroxy-Selenomethionine Mitigated Chronic Heat Stress-Induced Porcine Splenic Damage via Activation of Nrf2/Keap1 Signal and Suppression of NFκb and STAT Signal
Abstract
:1. Introduction
2. Results
2.1. Serum Immunoglobulin and Inflammatory Level
2.2. Spleen Mass, Se Deposition, HSP70 mRNA and Protein Expression
2.3. Splenic Antioxidant Capacity
2.4. The Protein Abundance Related to the NRF2 Signal
2.5. The Protein Expression of Apoptosis-Related Proteins
2.6. The Phosphorylation Levels of NFκb and STAT Signal
2.7. The mRNA Expression of Inflammatory Response-Related Genes
2.8. The mRNA Expression of Splenic Selenoproteins
2.9. The Proteins Expression of Key Response Splenic Selenoproteins
3. Discussion
4. Materials and Methods
4.1. Animal, Experiment Design and Management
4.2. Blood and Tissue Collection
4.3. Blood Inflammatory Factor and Immunoglobulin
4.4. Selenium Concentration in Spleen Tissue
4.5. Splenic Antioxidant Capacity Analyses
4.6. Real-Time PCR Analyses
4.7. Western Blot Analyses
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Item | Con | High-Temperature Conditions | SEM * | ANOVA p-Value | Linear | Quadratic | |||
---|---|---|---|---|---|---|---|---|---|
CHS | 0.2SeO | 0.4SeO | 0.6SeO | ||||||
IgA (mg/L) | 49.54 | 46.67 | 46.66 | 42.23 | 43.16 | 0.97 | 0.103 | 0.133 | 0.827 |
IgG (g/L) | 3.88 ab | 4.38a | 4.32 a | 3.57 ab | 3.03 b | 0.14 | 0.002 | <0.001 | 0.296 |
IgM (mg/L) | 0.40 | 0.41 | 0. 43 | 0.41 | 0.45 | 0.01 | 0.432 | 0.198 | 0.488 |
TNF-α (ng/L) | 0.41 | 0.43 | 0.36 | 0.45 | 0.45 | 0.01 | 0.115 | 0.233 | 0.228 |
IL-6 (ng/L) | 1.10 a | 0.94 ab | 1.08 a | 0.82 b | 0.97 ab | 0.27 | 0.009 | 0.963 | 0.358 |
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Liu, Y.; Yin, S.; He, Y.; Tang, J.; Pu, J.; Jia, G.; Liu, G.; Tian, G.; Chen, X.; Cai, J.; et al. Hydroxy-Selenomethionine Mitigated Chronic Heat Stress-Induced Porcine Splenic Damage via Activation of Nrf2/Keap1 Signal and Suppression of NFκb and STAT Signal. Int. J. Mol. Sci. 2023, 24, 6461. https://doi.org/10.3390/ijms24076461
Liu Y, Yin S, He Y, Tang J, Pu J, Jia G, Liu G, Tian G, Chen X, Cai J, et al. Hydroxy-Selenomethionine Mitigated Chronic Heat Stress-Induced Porcine Splenic Damage via Activation of Nrf2/Keap1 Signal and Suppression of NFκb and STAT Signal. International Journal of Molecular Sciences. 2023; 24(7):6461. https://doi.org/10.3390/ijms24076461
Chicago/Turabian StyleLiu, Yan, Shenggang Yin, Ying He, Jiayong Tang, Junning Pu, Gang Jia, Guangmang Liu, Gang Tian, Xiaoling Chen, Jingyi Cai, and et al. 2023. "Hydroxy-Selenomethionine Mitigated Chronic Heat Stress-Induced Porcine Splenic Damage via Activation of Nrf2/Keap1 Signal and Suppression of NFκb and STAT Signal" International Journal of Molecular Sciences 24, no. 7: 6461. https://doi.org/10.3390/ijms24076461