The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans
Abstract
:1. Introduction
2. Results
2.1. Effects of MI on the Lifespan in the C. elegans When the Osmotic Pressure Interference Was Excluded
2.2. Effects of MI on the Pharyngeal Pumping, Body Bend and Autofluorescence in the C. elegans When the Osmotic Pressure Interference Was Excluded
2.3. Effects of MI on the Lifespan, Health Indexes and Autofluorescence of the AKT-1 Mutants
2.4. Effects of MI on the Lifespan, Health Indexes and Autofluorescence of the DAF-16 Mutants
2.5. Effects of MI on the Lifespan, Health Indexes and Autofluorescence of the DAF-18 Mutants When the Osmotic Pressure Interference Was Excluded
2.6. Effects of MI on the Nuclear Localization of the DAF-16
2.7. Effects of MI on the Expression of PI3K and Phosphorylation of AKT in the C. elegans and the Hs68 Cells
2.8. Effects of MI on the PTEN Expressions in the Hs68 Cells
2.9. Effects of MI on the PI3K Activity In Vitro
2.10. Effects of MI on the PTEN Activities In Vitro
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Handling Procedures for the C. elegans
4.3. Synchronization of the C. elegans
4.4. Assay of the Lifespan of the C. elegans
4.5. Assay of the Pharyngeal Pumping in the C. elegans
4.6. Assay of the Body Bends in the C. elegans
4.7. Determination of the Autofluorescence in the C. elegans
4.8. Distinguishing the Loss-of-Function Mutants
4.9. DAF-16 Nuclear Localization Assay in the C. elegans
4.10. Western Blotting in the C. elegans and Hs68 Cells
4.11. Measurement of the Inhibition of MI on the PI3K Activity
4.12. In Vitro PTEN Activity Assay
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mean Lifespan (Day) | Median Lifespan (Day) | Maximum Lifespan (Day) | p Value * | |
---|---|---|---|---|
Control | 20.0 ± 5.6 | 22 | 30 | |
MI 25 nmol/plate | 22.0 ± 5.7 | 22 | 34 | 0.028 |
MI 50 nmol/plate | 23.0 ± 5.0 | 24 | 32 | <0.001 |
MI 100 nmol/plate | 24.4 ± 4.7 | 24 | 38 | <0.001 |
MI 200 nmol/plate | 22.1 ± 4.7 | 22 | 32 | 0.076 |
Mutants | Mean Lifespan (Day) | Median Lifespan (Day) | Maximum Lifespan (Day) | p Value * |
---|---|---|---|---|
AKT-1 Control MI | 21.9 ± 5.4 | 22 | 34 | |
22.0 ± 5.3 | 22 | 36 | 0.921 | |
DAF-16 Control MI | 14.9 ± 4.2 | 16 | 24 | |
14.4 ± 4.3 | 16 | 24 | 0.517 | |
DAF-18 Control MI | 14.2 ± 3.4 | 14 | 22 | |
13.4 ± 3.4 | 14 | 20 | 0.121 |
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Yang, N.-C.; Chin, C.-Y.; Zheng, Y.-X.; Lee, I. The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans. Int. J. Mol. Sci. 2023, 24, 6194. https://doi.org/10.3390/ijms24076194
Yang N-C, Chin C-Y, Zheng Y-X, Lee I. The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans. International Journal of Molecular Sciences. 2023; 24(7):6194. https://doi.org/10.3390/ijms24076194
Chicago/Turabian StyleYang, Nae-Cherng, Chia-Yu Chin, Ya-Xin Zheng, and Inn Lee. 2023. "The Attenuation of Insulin/IGF-1 Signaling Pathway Plays a Crucial Role in the Myo-Inositol-Alleviated Aging in Caenorhabditis elegans" International Journal of Molecular Sciences 24, no. 7: 6194. https://doi.org/10.3390/ijms24076194