DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload
Abstract
:1. Introduction
2. Results
2.1. LV Dilatation and Contractile Dysfunction in 16-Week-VO-Exposed Mice
2.2. Landscape of DNA Methylation in Volume-Overloaded Dilated LV
2.3. Validation of Promoter Methylation
2.4. Conserved Methylation Pattern in Circulating DNA
2.5. Association of Loci-Specific Differential Methylation with the Corresponding Gene Expression
2.6. Similar Methylation Changes Occurred in Dilated LV Early after Shunt before Functional Deterioration
2.7. No Major Changes in the Expression Levels of the DNA Methylation-Modifying Enzymes in Volume-Overloaded Dilated Remodeled LV
3. Discussion
Study Limitations
4. Materials and Methods
4.1. Aortocaval Shunt
4.2. Genomic DNA Isolation
4.3. Reduced Representation Bisulfite Sequencing (RRBS)
4.4. Data Processing and Mapping to the Mouse Genome
4.5. Methylated DNA Immunoprecipitation (MeDIP)-Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.6. Bisulfite Sequencing
4.7. Microarray Data Resources
4.8. Quantitative RT-PCR
4.9. Echocardiography
4.10. Histology
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
5mC | 5-methylcytosine |
Asmt | acetylserotonin O-methyltransferase |
Cdk5rap2 | CDK5 regulatory subunit-associated protein 2 |
CpG | Cytosine–guanine dinucleotides |
DMRs | differentially methylated regions |
DNMTs | DNA methyltransferases |
ER | endoplasmic reticulum |
Erdr1 | erythroid differentiation regulator 1 |
HF | heart failure |
Ipmk | inositol polyphosphate multikinase |
Jph2 | junctophilin-2 |
LV | left ventricle |
MeDIP | methylated DNA immunoprecipitation |
MI | myocardial infarction |
Nlrp5 | NLR family, pyrin domain containing 5 |
PO | pressure overload |
qRT-PCR | quantitative real-time polymerase chain reaction |
RRBS | reduced representation bisulfite sequencing |
Spcs3 | signal peptidase complex subunit 3 |
TET | ten-eleven translocation methylcytosine dioxygenases |
Vapb | vesicle-associated membrane protein-associated protein B |
VO | volume overload |
WGA | wheat germ agglutinin |
WT | wild type |
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Xu, X.; Elkenani, M.; Tan, X.; Hain, J.k.; Cui, B.; Schnelle, M.; Hasenfuss, G.; Toischer, K.; Mohamed, B.A. DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload. Int. J. Mol. Sci. 2023, 24, 5885. https://doi.org/10.3390/ijms24065885
Xu X, Elkenani M, Tan X, Hain Jk, Cui B, Schnelle M, Hasenfuss G, Toischer K, Mohamed BA. DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload. International Journal of Molecular Sciences. 2023; 24(6):5885. https://doi.org/10.3390/ijms24065885
Chicago/Turabian StyleXu, Xingbo, Manar Elkenani, Xiaoying Tan, Jara katharina Hain, Baolong Cui, Moritz Schnelle, Gerd Hasenfuss, Karl Toischer, and Belal A. Mohamed. 2023. "DNA Methylation Analysis Identifies Novel Epigenetic Loci in Dilated Murine Heart upon Exposure to Volume Overload" International Journal of Molecular Sciences 24, no. 6: 5885. https://doi.org/10.3390/ijms24065885