Oncolytic Effect of Zika Virus in Neuroendocrine Pancreatic Tumors: New Perspectives for Therapeutic Approaches
Abstract
:1. Introduction
2. Results
2.1. Cell Morphological Evaluation after ZIKV Infection
2.2. NCAM Expression in Pancreatic Tumor Cells
2.3. Evaluation of ZIKV Replication in NET Cells
2.4. Evaluation of Cell Growth, Cell Cycle, and Cell Death after ZIKV Infection
3. Discussion
4. Materials and Methods
4.1. Cell Culture, Transfection, and Infection
4.2. RNA Extraction and RT-PCR
4.3. Immunofluorescence
4.4. Immunoblotting
4.5. Propidium Iodide Cell Cycle Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diagnosis | Grading | Mitotic Index (HPF) * | Ki-67 (%) † | Morphology |
---|---|---|---|---|
NET | 1 | <2/10 | ≤3 | Well differentiated |
NET | 2 | 2–20/10 | 3–20 | Well differentiated |
NET | 3 | >20/10 | >20 | Well differentiated |
NEC - Small cell - Large cell | 3 | >20/10 | >20 | Poorly differentiated |
MiNEN | - | - | - | Well—Poor differentiated |
Tumor-like lesions | - | - | - | - |
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Cocco, M.M.; Carcione, C.; Miceli, V.; Tinnirello, R.; Chinnici, C.M.; Carbone, C.; Zito, G.; Conaldi, P.G.; Iannolo, G. Oncolytic Effect of Zika Virus in Neuroendocrine Pancreatic Tumors: New Perspectives for Therapeutic Approaches. Int. J. Mol. Sci. 2023, 24, 17271. https://doi.org/10.3390/ijms242417271
Cocco MM, Carcione C, Miceli V, Tinnirello R, Chinnici CM, Carbone C, Zito G, Conaldi PG, Iannolo G. Oncolytic Effect of Zika Virus in Neuroendocrine Pancreatic Tumors: New Perspectives for Therapeutic Approaches. International Journal of Molecular Sciences. 2023; 24(24):17271. https://doi.org/10.3390/ijms242417271
Chicago/Turabian StyleCocco, Martina Maria, Claudia Carcione, Vitale Miceli, Rosaria Tinnirello, Cinzia Maria Chinnici, Carmine Carbone, Giovanni Zito, Pier Giulio Conaldi, and Gioacchin Iannolo. 2023. "Oncolytic Effect of Zika Virus in Neuroendocrine Pancreatic Tumors: New Perspectives for Therapeutic Approaches" International Journal of Molecular Sciences 24, no. 24: 17271. https://doi.org/10.3390/ijms242417271