Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues
Abstract
:1. Introduction
2. Results
2.1. Transcript Abundances of VDR, PDIA3, CYP27C1, and CYP24A1 mRNA in the Hen Reproductive System
2.2. Abundances of VDR, PDIA3, 1α-hydroxylase, and 24-hydroxylase Protein in the Hen Reproductive System
2.3. Immunofluorescent Localization of PDIA3, 1α-hydroxylase, and 24-hydroxylase in the Hen Reproductive System
3. Discussion
4. Materials and Methods
4.1. Chickens and Tissue Sampling
4.2. RNA Isolation and qRT-PCR
4.3. Western Blot Analysis
4.4. Immunofluorescence
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | GeneBank Identification | Assay ID | Amplicon Size [bp] |
---|---|---|---|
VDR | NM_205098.1 | Gg03348521_g1 | 80 |
PDIA3 | NM_204110.3 | Gg03346843_m1 | 90 |
CYP27C1 | XM_004942891.3 | Gg03326395_m1 | 107 |
CYP24A1 | AF019142.1 | Gg03347931_m1 | 90 |
18S rRNA | X03205.1 | Cat. no. 4310893E | 187 |
Antibody | Serum | Host Species | Vendor | Cat. No | WB Dilution | IF Dilution |
---|---|---|---|---|---|---|
Anti-VDR | 5% NGS | Rabbit | Cell Signaling, Massachusetts, DA, USA | 12550 | 1:1000 | 1:50 |
Anti-PDIA3 | 5% NGS | Rabbit | Proteintech, Manchester, UK | 15967-1-AP | 1:5000 | 1:200 |
1α-hydroxylase | 5% NGS | Rabbit | Invitrogen, Carsband, CA, USA | PA5-79128 | 1:2000 | 1:200 |
24-hydroxylase | 5% NGS | Rabbit | Invitrogen, Carsband, CA, USA | PA5-79127 | 1:1000 | 1:150 |
Anti-β-actin | - | Mouse | Sigma-Aldrich, St. Louis, MO, USA | A2228 | 1:4000 | - |
Anti-vinculin | - | Mouse | Sigma-Aldrich, St. Louis, MO, USA | V9264 | 1:2000 | - |
HRP-anti mouse IgG | - | Horse | Bio-Rad Laboratories Inc., GmbH, Munich, Germany | 170-6516 | 1:3000 | - |
HRP-anti rabbit IgG | - | Goat | Invitrogen, Carsband, CA, USA | 31460 | 1:3000 | - |
DyLight 594-anti rabbit | 5% NGS | Goat | Vector Laboratories, Burlingame, CA, USA | DI-1594-1.5 | - | 1:150 |
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Hrabia, A.; Kamińska, K.; Socha, M.; Grzesiak, M. Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues. Int. J. Mol. Sci. 2023, 24, 17074. https://doi.org/10.3390/ijms242317074
Hrabia A, Kamińska K, Socha M, Grzesiak M. Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues. International Journal of Molecular Sciences. 2023; 24(23):17074. https://doi.org/10.3390/ijms242317074
Chicago/Turabian StyleHrabia, Anna, Kinga Kamińska, Magdalena Socha, and Małgorzata Grzesiak. 2023. "Vitamin D3 Receptors and Metabolic Enzymes in Hen Reproductive Tissues" International Journal of Molecular Sciences 24, no. 23: 17074. https://doi.org/10.3390/ijms242317074