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Volume 24
Issue 21
10.3390/ijms242115932
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Article
Peer-Review Record

Human Synovial Mesenchymal Stem Cells Expressed Immunoregulatory Factors IDO and TSG6 in a Context of Arthritis Mediated by Alphaviruses

Int. J. Mol. Sci. 2023, 24(21), 15932; https://doi.org/10.3390/ijms242115932
by Melissa Payet 1, Axelle Septembre-Malaterre 1, Philippe Gasque 1,2 and Xavier Guillot 1,3,*
Reviewer 1: Anonymous
Reviewer 2:
Ankita Singh
Int. J. Mol. Sci. 2023, 24(21), 15932; https://doi.org/10.3390/ijms242115932
Submission received: 29 September 2023 / Revised: 26 October 2023 / Accepted: 1 November 2023 / Published: 3 November 2023
(This article belongs to the Section Molecular Immunology)

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

Dear authors, 

I'm afraid that the in vitro experiment did not mimic a chronic inflammatory process. To that, more than 24 hours would be necessary to evaluate a long term cell system alteration. 

In addition, I could not find any citation on the literature for the miRs analyzed besides your review in 2021, which states that the samples were "human samples" and not specific MSCs. How do you explain that? Why do not analyze others miRs not connect with tumor development ? 

Also, was the media containing TNF or IFN changed to a fresh one w/o cytokines before the virus addition? Was the virus added into a media with cytokines at any time point? 

Why do not test MSCs function after the infection? Are they anti or proinflammatory after the infection? 

I do not agree with the title as the cell culture was not performed to reproduce a chronic arthritis model. 

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The study shows that in response to ONNV infection, the MSC produce pro-inflammatory but also regulatory response during chronic phase of inflammation. Resulting in incomplete clearance of the virus. However, the authors have used just 24 h culture to mimic chronic inflammatory milieu and show induction of immune regulatory response. Please provide the notion behind the planning of the experiment.

A correlation between increase in anti-inflammatory response with inflammatory response with time will give a better picture about virus infection mediated changes.

The data shows that both anti and pro-inflammatory response go down by 24 h except for expression of miR-146. Please discussion the context in the discussion.

Figure 2 please provide scale bar and quantify Ki67+ cells.

Line-252 TSG6?

Line-309 in TSG6

Line-318 de TSG6

Comments on the Quality of English Language

Minor editing

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Comments and Suggestions for Authors

Dear authors

I see and understand that you made all required modifications by both reviewers. No major comments from my side. 

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