Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases’ Thermostability
Abstract
:1. Introduction
2. Sucrose Isomerases, Structure and Reaction Mechanism
3. Thermolability of Sucrose Isomerases
4. Protein Engineering, Thermostabilization of Sucrose Isomerases
4.1. Directed Evolution
4.2. Rational Design
4.3. Semi-Rational Design
4.4. Characterization of Thermostability
4.5. Thermostabilization of Sucrose Isomerases
5. Glycosylation of Sucrose Isomerases
6. Future Perspectives
6.1. Thermostable Sucrose Isomerases Based on Homology Models and Chimerization
6.2. Thermostable Sucrose Isomerases Based on Improving the Entropy of the Folded State
6.3. Thermostable Sucrose Isomerases, Other Alternative Strategies
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Microbial Source | Sucrose Isomerase | Mutation | PDB ID | Interacted Chemical | Resolution (Å) | References |
---|---|---|---|---|---|---|
Pseudomonas mesoacidophila MX-45 (Rhizobium sp. MX-45) | MutB | Non-mutant | 2PWH | native | 2.0 | [56] |
Non-mutant | 1ZJA | MutB-Tris | 1.6 | |||
Non-mutant | 2PWD | MutB-deoxynojirimycin | 1.8 | |||
Non-mutant | 2PWG | MutB-castanospermine | 2.2 | |||
D200A | 2PWF | MutB-glucose | 1.8 | |||
E254Q | 2PWE | MutB-sucrose | 2.0 | |||
Non-mutant | 1ZJB | MutB-Tris | 1.8 | [57] | ||
A258V | 4GO8 | MutB-Tris | 2.15 | |||
D415N | 4GO9 | MutB-Tris | 2.2 | |||
D200A-D415N (inactive enzyme) | 4HA1 | MutB-isomaltulose-glucose-Ca2+ | 2.2 | [58] | ||
Non-mutant | 4H8V | MutB-trehalulose-Ca2+ | 1.95 | |||
D200A-D415N (inactive enzyme) | 4H8U | MutB-trehalulose-glycerol-Ca2+ | 2.0 | |||
E254Q-D415N (inactive enzyme) | 4H8H | MutB-SO42−-glycerol-Ca2+ | 2.0 | |||
D200A-D415N (inactive enzyme) | 4H7V | MutB-glycerol-glucose-Ca2+ | 1.8 | |||
R284C | 4H2C | MutB-glycerol-Ca2+ | 1.7 | |||
R284C | 4GIN | MutB-glycerol-Ca2+ | 1.9 | |||
F164L | 4GIA | MutB-Tris-glycerol-Ca2+ | 2.01 | |||
F164L | 4GI9 | MutB-Tris-glycerol-Ca2+ | 2.15 | |||
F164L | 4GI8 | MutB-Tris-glycerol-Ca2+ | 1.95 | |||
F164L | 4GI6 | MutB-Tris-glycerol-glucose-Ca2+ | 2.15 | |||
Erwinia rhapontici NX-5 | NX-5 | Non-mutant | 4HOW | (NX-5)-glycerol-Ca2+ | 1.7 | [59] |
Non-mutant | 4HOX | (NX-5)-Tris-glycerol-Ca2+ | 2.0 | |||
D241A | 4HOZ | (NX-5)-glucose-glycerol-Ca2+ | 2.0 | |||
E295A | 4HP5 | (NX-5)-glucose-glycerol-Ca2+ | 2.0 | |||
E295Q | 4HPH | (NX-5)-sucrose-glycerol-Ca2+ | 1.7 | |||
Protaminobacter rubrum CBS574.77 | SmuA | Non-mutant | 3GBD | SmuA-C6H5O73−-ethylene glycol | 1.95 | [60] |
Non-mutant | 3GBE | SmuA-C6H5O73−-ethylene glycol-deoxynojirimycin | 1.7 | |||
Klebsiella sp. LX3 | PalI | Non-mutant | 1M53 | No information | 2.2 | [61] |
Isoform | Top (°C) | pH | Specific Activity (U/mg) | Km (mM) | kcat/Km (mM−1·s−1) | Isomaltulose Ratio (%) | References |
---|---|---|---|---|---|---|---|
Wild-type PalI NX-5 | 30 | 6 | 423 | 222 | NR | 83 | [87] |
Recombinant PalI NX-5 | 30 | 5 | NR | 257 | NR | 87 | [39] |
Recombinant PalI NX-5 | 30 | 6 | 483.8 | 255.1 | 2.2 | 78 | [54] |
Wild-type PalI D12 | 40 | 6 | 19.8 | 138 | NR | 65.7 | [40] |
Wild-type NCPPB 1578 | 30 | NR | 4.11 a | 280 | NR | 85 | [88] |
Recombinant CBS 574.77 | 35 | NR | NR | 32.4 | 1301 | 88.5 | [86] |
Recombinant PalI NK33 | 35 | 6 | 2362 | 42.7 | NR | 76.8 | [49] |
Recombinant UQ68J | 35 | 5 | 562 | 39.9 | 17.9 | 91 | [51] |
Recombinant UQ14S | 35 | 6 | 351 | 76 | 6.2 | 66 | [51] |
Recombinant PalI LX3 | 35 | 6 | 328 | 54.6 | 0.27 | 83 | [85] |
Wild-type ATCC15928 | 30 | 6.2 | 120 | 65 | NR | 72.6 | [41] |
Recombinant AS9 | 30 | 6 | 957.5 | 30.1 | 33 | 76.3 | [55] |
Recombinant Ejp617 | 40 | 6 | 118.87 | 69.28 | NR | 80.5 | [89] |
Recombinant FMB-1 | 50 | 5–6 | 49 | NR | NR | 78 | [50] |
Recombinant Pal-2 | 40 | 5.5 | 286.4 | 62.9 | NR | 81.7 | [48] |
Isoform | Modification | Strategy for Thermostabilization | Stabilizing Interaction | Top (°C) | Half-Life (min) | Specific Activity (U/mg) | Km (mM) | kcat/Km (mM−1·s−1) | References |
---|---|---|---|---|---|---|---|---|---|
PalI NX-5 | Glycosylation | B-factor analysis and glycosylation site engineering. | Strengthening of the hydrogen-bonding network and glycosylation of the flexible terminal C region. | 30 | 10.1 a | 483.8 | 255.1 | 2.21 | [54] |
PalI NX-5 | Glycosylation—K174Q | 35 | 22.3 a | 529.9 | 241.9 | 2.55 | |||
PalI NX-5 | Glycosylation—L202E | 35 | 17.5 a | 509.1 | 234.9 | 2.52 | |||
PalI NX-5 | Glycosylation—K174Q/L202E | 35 | 29.2 a | 509.3 | 231.2 | 2.57 | |||
PalI AS9 | Native | B-factor analysis | Strengthening of the hydrogen bridge network | 30 | 39.2 b | 957.5 | 30.1 | 33 | [55] |
PalI AS9 | E175N | 35 | 90.2 b | 1017.6 | 28.1 | 45.6 | |||
PalI AS9 | K576D | 35 | 69.8 b | 1045.7 | 29.5 | 34.4 | |||
PalI AS9 | E175N/K576D | 35 | 300 b | 1218.9 | 26.8 | 39.4 | |||
PalI LX3 | Native | Proline theory | Loop stabilization | 35 | 1.81 c | 328 | 54.6 | 0.27 | [85] |
PalI LX3 | E498P | 40 | 9.45 c | 350 | NR | 0.29 | |||
PalI LX3 | E498P/R310P | 40 | 13.61 c | 340 | NR | 0.31 |
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Sardiña-Peña, A.J.; Mesa-Ramos, L.; Iglesias-Figueroa, B.F.; Ballinas-Casarrubias, L.; Siqueiros-Cendón, T.S.; Espinoza-Sánchez, E.A.; Flores-Holguín, N.R.; Arévalo-Gallegos, S.; Rascón-Cruz, Q. Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases’ Thermostability. Int. J. Mol. Sci. 2023, 24, 14513. https://doi.org/10.3390/ijms241914513
Sardiña-Peña AJ, Mesa-Ramos L, Iglesias-Figueroa BF, Ballinas-Casarrubias L, Siqueiros-Cendón TS, Espinoza-Sánchez EA, Flores-Holguín NR, Arévalo-Gallegos S, Rascón-Cruz Q. Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases’ Thermostability. International Journal of Molecular Sciences. 2023; 24(19):14513. https://doi.org/10.3390/ijms241914513
Chicago/Turabian StyleSardiña-Peña, Amado Javier, Liber Mesa-Ramos, Blanca Flor Iglesias-Figueroa, Lourdes Ballinas-Casarrubias, Tania Samanta Siqueiros-Cendón, Edward Alexander Espinoza-Sánchez, Norma Rosario Flores-Holguín, Sigifredo Arévalo-Gallegos, and Quintín Rascón-Cruz. 2023. "Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases’ Thermostability" International Journal of Molecular Sciences 24, no. 19: 14513. https://doi.org/10.3390/ijms241914513