DHA-Provoked Reduction in Adipogenesis and Glucose Uptake Could Be Mediated by Gps2 Upregulation in Immature 3T3-L1 Cells
Abstract
:1. Introduction
2. Results
2.1. Pre-Experimental Procedure
2.2. The Effect of DHA on Adipogenesis
2.3. The Effect of DHA on Cell Number and Viability at the End of the Experiment
2.4. The Effect of DHA on Lipolysis
2.5. Carnitine Palmitoyltransferase 1 (Cpt1) Gene Expression
2.6. The Effect of DHA on Glucose Uptake
3. Discussion
4. Materials and Methods
4.1. Cell Culture Handling
4.2. Fatty Acid Dissolving Procedure
4.3. Pre-Experimental Procedure: Cell Viability Assays
4.4. Experimental Design
4.5. Assessment of Neutral Lipid Accumulation
4.6. Lipolysis Rate Evaluation
4.7. Glucose Consumption Estimation
4.8. Gene Expression Analyses
4.9. ELISA Adiponectin Quantification
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abbreviation | Full Name | Forward Primer | Reverse Primer | Product Size (bp) |
---|---|---|---|---|
Pparg NM_001127330.2 | Peroxisome proliferator-activated receptor gamma, transcript variant 2 | AGGGCGATCTTGACAGGAAA | CGAAACTGGCACCCTTGAAA | 164 |
Gpr120 NM_181748.2 | G protein-coupled receptor 120 | CCAACCGCATAGGAGAAATC | CAAGCTCAGCGTAAGCCTCT | 140 |
Fasn NM_007988.3 | Fatty acid synthase | CTGAAGCCGAACACCTCTGT | GGGAATGTTACACCTTGCTCCT | 218 |
Acaca NM_133360.3 | Acetyl-CoA carboxylase | TGCTCATGTTCCTTGCCCAA | TGCCACCACCATATTTGAGATT | 247 |
Cpt1 NM_153679.2 | Carnitine palmitoyltransferase 1 | GTGTACTTCCAACTACGTCAGC | GCGATACAGGAGCAGGGTAT | 169 |
Gps2 NM_001357906.2 | G protein pathway suppressor 2 | ACAAGTGCTTACGACCCGG | GGAAATGCTGATGGGGCTCT | 183 |
Pik3r1 NM_001077495.2 | Phosphoinositide-3-kinase regulatory subunit 1 | ATACTTGATGTGGCTGACGC | GTCTCGCTTCCCTCGCAATA | 193 |
Slc2a4 NM_009204.2 | Solute carrier family 2 member 4 | CGTTGGTCTCGGTGCTCTTA | AGCTCTGCCACAATGAACCA | 220 |
Hprt NM_013556.2 | Hypoxanthine guanine phosphoribosyl transferase | ACAGGCCAGACTTTGTTGGA | ACTTGCGCTCATCTTAGGCT | 150 |
Actb NM_007393.5 | β-actin | CCTCTATGCCAACACAGTGC | GTACTCCTGCTTGCTGATCC | 211 |
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Grigorova, N.; Ivanova, Z.; Vachkova, E.; Petrova, V.; Penev, T. DHA-Provoked Reduction in Adipogenesis and Glucose Uptake Could Be Mediated by Gps2 Upregulation in Immature 3T3-L1 Cells. Int. J. Mol. Sci. 2023, 24, 13325. https://doi.org/10.3390/ijms241713325
Grigorova N, Ivanova Z, Vachkova E, Petrova V, Penev T. DHA-Provoked Reduction in Adipogenesis and Glucose Uptake Could Be Mediated by Gps2 Upregulation in Immature 3T3-L1 Cells. International Journal of Molecular Sciences. 2023; 24(17):13325. https://doi.org/10.3390/ijms241713325
Chicago/Turabian StyleGrigorova, Natalia, Zhenya Ivanova, Ekaterina Vachkova, Valeria Petrova, and Toncho Penev. 2023. "DHA-Provoked Reduction in Adipogenesis and Glucose Uptake Could Be Mediated by Gps2 Upregulation in Immature 3T3-L1 Cells" International Journal of Molecular Sciences 24, no. 17: 13325. https://doi.org/10.3390/ijms241713325