Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian
Abstract
:1. Introduction
2. Results
2.1. Preparation of ZJ1 Extracts
2.2. Anti-Melanogenic Effect of ZJ1 Extracts on B16-F10 Cells
2.3. BL and WL Reduce Intracellular Tyrosinase Activity
2.4. BL and WL Downregulate Melanogenesis-Related Gene Expression
2.5. BL and WL Inhibit Melanogenesis in Zebrafish Embryos
2.6. Antioxidant Property of ZJ1 Extracts
3. Discussion
4. Materials and Methods
4.1. Materials and Reagents
4.2. Preparation of Bacterial Lysates, Whole Lysates, Cell-Free Supernatant and Polysaccharides
4.3. Cell Culture
4.4. Cell Viability Assay
4.5. Assay of Melanin Contents in Melanoma Cells B16-F10
4.6. Assay of Cell-Free Mushroom Tyrosinase Activity
4.7. Assay of Intracellular Tyrosinase Activity
4.8. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Quantitative Real-Time Polymerase Chain Reaction
4.9. ABTS Assay
4.10. Zebrafish Maintenance and Embryo Collection
4.11. Assay of Melanin Content and Tyrosinase Activity in Zebrafish Embryos
4.12. Assay of Cellular Reactive Oxygen Species (ROS) in Zebrafish Embryo Model
4.13. Statistics
5. Conclusions
6. Limitations of the Study
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Fraction | Bacterial Lysate (BL) (mg/g, n = 9) | Cell-Free Supernatant (CFS) (mg/mL, n = 5 or 8) | Whole Lysate (WL) (mg/mL, n = 7) |
---|---|---|---|
Polysaccharide | n.d | 0.16 ± 0.03 | n.d |
Protein | 9.56 ± 1.12 | 11.82 ± 0.94 | 11.75 ± 0.93 |
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Wu, J.; Zhang, F.; Yu, H.; Qi, S.; Wu, Y.; Xiao, W. Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian. Int. J. Mol. Sci. 2023, 24, 12810. https://doi.org/10.3390/ijms241612810
Wu J, Zhang F, Yu H, Qi S, Wu Y, Xiao W. Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian. International Journal of Molecular Sciences. 2023; 24(16):12810. https://doi.org/10.3390/ijms241612810
Chicago/Turabian StyleWu, Jing, Funa Zhang, Haixia Yu, Shimei Qi, Yu Wu, and Weihua Xiao. 2023. "Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian" International Journal of Molecular Sciences 24, no. 16: 12810. https://doi.org/10.3390/ijms241612810