Influence of Electrospun Fibre Secondary Morphology on Antibiotic Release Kinetic and Its Impact on Antimicrobic Efficacy
Abstract
:1. Introduction
2. Results
2.1. Polymer Solutions Characterization
2.2. Morphological Characterization of Small-Diameter Vascular Grafts Prototypes
2.3. Small-Diameter Vascular Graft Prototypes Hydration Profiles
2.4. In Vitro Drug Release Profiles and Kinetics of Small-Diameter Vascular Graft Prototypes
2.5. Drug Release Kinetic Evaluation for Vasculas Graft Prototypes
2.6. Antimicrobic Activity of Small-Diameter Vascular Graft Prototypes
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Polymer Solutions Preparation and Characterization
4.2.2. Vascular Graft Prototypes Electrospinning and Morphological Characterization
4.2.3. Water up Take Assay
4.2.4. Drug Encapsulation Efficiency, Vascular Grafts Drug Content and In Vitro Drug Release Evaluation
4.2.5. Drug Release Kinetic Study
4.2.6. Antimicrobic Assay
4.2.7. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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PLGA Concentration (% w/v) | Voltage (kV) | Flow Rate (mL/h) | Temperature Range (°C) | Relative Humidity (%) |
---|---|---|---|---|
10 | 18 | 0.1 | 30–35 | 20 |
12.5 | 20 | 0.3 | 45–37 | 20 |
Graft Type | Wall Thickness (μm) | Fibre Diameter (μm) | Pore Diameter (μm) | Porosity (%) |
---|---|---|---|---|
PR1_PL | 291.95 ± 32.64 | 1.784 ± 0.561 | 17.20 ± 5.24 | 43.79 ± 0.90 |
PR1_T | 291.00 ± 20.82 | 1.224 ± 0.301 | 16.00 ± 4.691 | 45.82 ± 0.44 |
PR2_PL | 180.00 ± 18.00 | 0.794 ± 0.178 | 9.91 ± 3.27 | 44.00 ± 2.64 |
PR2_T | 167.10 ± 13.08 | 0.870 ± 0.248 | 10.96 ± 3.00 | 44.49 ± 3.77 |
Mathematical Model | Prototype 1 | Prototype 2 | ||||||
---|---|---|---|---|---|---|---|---|
R2 | R2-adj | AIC | MSC | R2 | R2-adj | AIC | MSC | |
Higuchi | −0.2953 | −0.2953 | 203 | −0.3540 | 0.9386 | 0.9386 | 138 | 2.6959 |
Korsmeyer–Peppas | 0.9332 | 0.9297 | 143 | 2.5158 | 0.9886 | 0.9874 | 107 | 4.1912 |
Peppas–Sahlin | 0.8304 | 0.8116 | 164 | 1.4887 | 0.9909 | 0.9905 | 100 | 4.5131 |
Weibull | 0.9978 | 0.9891 | 108 | 4.1387 | 0.9643 | 0.9580 | 133 | 2.9507 |
Logistic | 0.9887 | 0.9874 | 109 | 4.1947 | 0.9276 | 0.9195 | 146 | 2.3396 |
Gompertz | 0.9834 | 0.9816 | 116 | 3.8131 | 0.8914 | 0.8794 | 154 | 1.9348 |
Probit | 0.9899 | 0.9888 | 105 | 4.4096 | 0.9356 | 0.9285 | 143 | 2.4576 |
Prototype | Best Fitting Model | Equation | Parameters |
---|---|---|---|
PR1 | Weibull | F = Fmax × (1 − e[−((t − Ti)^β)/α]) | α = 2.557 ± 1.250 β = 0.295 ± 0.102 Ti = 1.500 ± 0.329 |
PR2 | Peppas–Sahlin | F = k1 × tm + k2 × t(2m) | k1 = 5.233 ± 2.966 k2 = −0.069 ± 0.149 m = 0.45 |
Time (h) | ME | ME |
---|---|---|
S. aureus | E. coli | |
24 | 3.98 ± 1.47 | 5.89 ± 1.16 |
48 | 6.31 ± 0.41 | 8.82 ± 0.07 |
72 | 6.30 ± 0.15 | 8.42 ± 0.40 |
96 | 7.55 ± 1.50 | 8.15 ± 0.53 |
120 | 8.58 ± 0.05 | 8.39 ± 0.31 |
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Rosalia, M.; Grisoli, P.; Dorati, R.; Chiesa, E.; Pisani, S.; Bruni, G.; Genta, I.; Conti, B. Influence of Electrospun Fibre Secondary Morphology on Antibiotic Release Kinetic and Its Impact on Antimicrobic Efficacy. Int. J. Mol. Sci. 2023, 24, 12108. https://doi.org/10.3390/ijms241512108
Rosalia M, Grisoli P, Dorati R, Chiesa E, Pisani S, Bruni G, Genta I, Conti B. Influence of Electrospun Fibre Secondary Morphology on Antibiotic Release Kinetic and Its Impact on Antimicrobic Efficacy. International Journal of Molecular Sciences. 2023; 24(15):12108. https://doi.org/10.3390/ijms241512108
Chicago/Turabian StyleRosalia, Mariella, Pietro Grisoli, Rossella Dorati, Enrica Chiesa, Silvia Pisani, Giovanna Bruni, Ida Genta, and Bice Conti. 2023. "Influence of Electrospun Fibre Secondary Morphology on Antibiotic Release Kinetic and Its Impact on Antimicrobic Efficacy" International Journal of Molecular Sciences 24, no. 15: 12108. https://doi.org/10.3390/ijms241512108