Figure 1.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) for 2 h (TNM 2h), 5 h (TNM 5h), 15 h (TNM 15h), and 24 h (TNM 24h). After 15 h of contact, a complete inactivation of the virus is observed.
Figure 1.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) for 2 h (TNM 2h), 5 h (TNM 5h), 15 h (TNM 15h), and 24 h (TNM 24h). After 15 h of contact, a complete inactivation of the virus is observed.
Figure 2.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), 5 h (TNM+S 5h), 15 h (TNM+S 15h), and 24 h (TNM+S 24h). After 5 h of contact, a complete inactivation of the viral activity is noticed.
Figure 2.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), 5 h (TNM+S 5h), 15 h (TNM+S 15h), and 24 h (TNM+S 24h). After 5 h of contact, a complete inactivation of the viral activity is noticed.
Figure 3.
Reduction of phi 6 infection titers in the decadic logarithm of plaque-forming units per mL (LOG PFU/mL) measured by the double-layer method. Bacteriophages in contact with tiger nut milk (TNM) concentrated at 10X of initial concentration for 2 h (TNM 10X 2h), TNM concentrated at 10X of initial concentration for 5 h (TNM 10X 5h), TNM concentrated at 10X of initial concentration for 15 h (TNM 10X 15h), and TNM concentrated at 10X of initial concentration for 24 h (TNM 10X 24h). TNM concentrated at 0.1X of initial concentration for 2 h (TNM 0.1X 2h), bacteriophages in contact with TNM diluted at 0.1X of initial concentration for 5 h (TNM 0.1X 5h), TNM diluted at 0.1X of initial concentration for 15 h (TNM 0.1X 15h), and TNM diluted at 0.1X of initial concentration for 24 h (TNM 0.1X 24h). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001. A significant antiviral activity against bacteriophage phi 6 is achieved after only 5 h of contact with the 10X concentrated form of TNM. The 0.1X diluted form of TNM shows no antiviral properties after 24 h of contact.
Figure 3.
Reduction of phi 6 infection titers in the decadic logarithm of plaque-forming units per mL (LOG PFU/mL) measured by the double-layer method. Bacteriophages in contact with tiger nut milk (TNM) concentrated at 10X of initial concentration for 2 h (TNM 10X 2h), TNM concentrated at 10X of initial concentration for 5 h (TNM 10X 5h), TNM concentrated at 10X of initial concentration for 15 h (TNM 10X 15h), and TNM concentrated at 10X of initial concentration for 24 h (TNM 10X 24h). TNM concentrated at 0.1X of initial concentration for 2 h (TNM 0.1X 2h), bacteriophages in contact with TNM diluted at 0.1X of initial concentration for 5 h (TNM 0.1X 5h), TNM diluted at 0.1X of initial concentration for 15 h (TNM 0.1X 15h), and TNM diluted at 0.1X of initial concentration for 24 h (TNM 0.1X 24h). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001. A significant antiviral activity against bacteriophage phi 6 is achieved after only 5 h of contact with the 10X concentrated form of TNM. The 0.1X diluted form of TNM shows no antiviral properties after 24 h of contact.
Figure 4.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with concentrated tiger nut milk (TNM 10X) for 2 h (TNM 10X 2h); concentrated TNM 10X for 5 h (TNM 10X 5h); concentrated TNM 10X for 15 h (TNM 10X 15h); concentrated TNM 10X for 24 h (TNM 10X 24h); diluted TNM 0.1X for 2 h (TNM 0.1X 2h); diluted TNM 0.1X for 5 h (TNM 0.1X 5h); diluted TNM 0.1X for 15 h (TNM 0.1X 15h), and diluted TNM 0.1X for 24 h (TNM 0.1X 24h). Antiviral activity is shown after only 2 h of contact with a 10X concentrated form of TNM, increasing this activity as time of contact increases, too. No antiviral activity is observed with the 0.1X diluted form of TNM.
Figure 4.
Loss of bacteriophage phi 6 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with concentrated tiger nut milk (TNM 10X) for 2 h (TNM 10X 2h); concentrated TNM 10X for 5 h (TNM 10X 5h); concentrated TNM 10X for 15 h (TNM 10X 15h); concentrated TNM 10X for 24 h (TNM 10X 24h); diluted TNM 0.1X for 2 h (TNM 0.1X 2h); diluted TNM 0.1X for 5 h (TNM 0.1X 5h); diluted TNM 0.1X for 15 h (TNM 0.1X 15h), and diluted TNM 0.1X for 24 h (TNM 0.1X 24h). Antiviral activity is shown after only 2 h of contact with a 10X concentrated form of TNM, increasing this activity as time of contact increases, too. No antiviral activity is observed with the 0.1X diluted form of TNM.
Figure 5.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Bacteriophages in contact with tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) for 2 h (TNM 2h), TNM for 5 h (TNM 5h), TNM for 15 h (TNM 15h), and TNM for 24 h (TNM 24h). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: * p < 0.05; ** p < 0.01; *** p < 0.001. No significant antiviral activity is observed after 24 h against bacteriophage MS2 with TNM at commercial concentration.
Figure 5.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Bacteriophages in contact with tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) for 2 h (TNM 2h), TNM for 5 h (TNM 5h), TNM for 15 h (TNM 15h), and TNM for 24 h (TNM 24h). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: * p < 0.05; ** p < 0.01; *** p < 0.001. No significant antiviral activity is observed after 24 h against bacteriophage MS2 with TNM at commercial concentration.
Figure 6.
Loss of MS2 bacteriophage viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) for 2 h (TNM 2h), TNM for 5 h (TNM 5h), TNM for 15 h (TNM 15h), and TNM for 24 h (TNM 24h).
Figure 6.
Loss of MS2 bacteriophage viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) for 2 h (TNM 2h), TNM for 5 h (TNM 5h), TNM for 15 h (TNM 15h), and TNM for 24 h (TNM 24h).
Figure 7.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. MS2 bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), TNM with sugar for 5 h (TNM+S 5h), TNM with sugar for 15 h (TNM+S 15h), and TNM with sugar for 24 h (TNM+S 24h). Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001. A significant reduction of viral activity is observed after 24 h against bacteriophage MS2 with TNM with sugar.
Figure 7.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. MS2 bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), TNM with sugar for 5 h (TNM+S 5h), TNM with sugar for 15 h (TNM+S 15h), and TNM with sugar for 24 h (TNM+S 24h). Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001. A significant reduction of viral activity is observed after 24 h against bacteriophage MS2 with TNM with sugar.
Figure 8.
Loss of bacteriophage MS2 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), TNM with sugar for 5 h (TNM+S 5h), TNM with sugar for 15 h (TNM+S 15h), and TNM with sugar for 24 h (TNM+S 24h). TNM with sugar shows antiviral properties against bacteriophage MS2 after 24 h of contact.
Figure 8.
Loss of bacteriophage MS2 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plate images of bacteriophages in contact with tiger nut milk (TNM) with sugar for 2 h (TNM+S 2h), TNM with sugar for 5 h (TNM+S 5h), TNM with sugar for 15 h (TNM+S 15h), and TNM with sugar for 24 h (TNM+S 24h). TNM with sugar shows antiviral properties against bacteriophage MS2 after 24 h of contact.
Figure 9.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. MS2 bacteriophages in contact with tiger nut milk (TNM) concentrated at 10X of initial concentration for 2 h (TNM 10X 2h), TNM concentrated at 10X of initial concentration for 5 h (TNM 10X 5h), TNM concentrated at 10X of initial concentration for 15 h (TNM 10X 15h), and TNM concentrated at 10X of initial concentration for 24 h (TNM 10X 24h). MS2 bacteriophages in contact with TNM diluted at 0.1X of initial concentration for 2 h (TNM 0.1X 2h), TNM diluted at 0.1X of initial concentration for 5 h (TNM 0.1X 5h), TNM diluted at 0.1X of initial concentration for 15 h (TNM 0.1X 15h), and TNM diluted at 0.1X of initial concentration for 24 h (TNM 0.1X 24h) were also tested. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001; ** p < 0.01; * p < 0.05. The 10X concentrated form and 0.1X diluted form of TNM showed no antiviral effect against bacteriophage MS2 after 24 h of contact.
Figure 9.
Reduction of infection titers of bacteriophage MS2 in the decadic logarithm of plaque-forming units per mL (LOG 10 PFU/mL) measured by the double-layer method. Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. MS2 bacteriophages in contact with tiger nut milk (TNM) concentrated at 10X of initial concentration for 2 h (TNM 10X 2h), TNM concentrated at 10X of initial concentration for 5 h (TNM 10X 5h), TNM concentrated at 10X of initial concentration for 15 h (TNM 10X 15h), and TNM concentrated at 10X of initial concentration for 24 h (TNM 10X 24h). MS2 bacteriophages in contact with TNM diluted at 0.1X of initial concentration for 2 h (TNM 0.1X 2h), TNM diluted at 0.1X of initial concentration for 5 h (TNM 0.1X 5h), TNM diluted at 0.1X of initial concentration for 15 h (TNM 0.1X 15h), and TNM diluted at 0.1X of initial concentration for 24 h (TNM 0.1X 24h) were also tested. Three independent antiviral tests were performed on two different days (n = 6). Significant differences with respect to control were determined by one-way ANOVA with Tukey’s correction for multiple comparisons: *** p < 0.001; ** p < 0.01; * p < 0.05. The 10X concentrated form and 0.1X diluted form of TNM showed no antiviral effect against bacteriophage MS2 after 24 h of contact.
Figure 10.
Loss of bacteriophage MS2 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plates of bacteriophages in contact with concentrated tiger nut milk (TNM 10X) for 2 h (TNM 10X 2h); concentrated TNM 10X for 5 h (TNM 10X 5h); concentrated TNM 10X for 15 h (TNM 10X 15h); concentrated TNM 10X for 24 h (TNM 10X 24h); diluted TNM 0.1X for 2 h (TNM 0.1X 2h); diluted TNM 0.1X for 5 h (TNM 0.1X 5h); diluted TNM 0.1X for 15 h (TNM 0.1X 15h) and diluted TNM 0.1X for 24 h (TNM 0.1X 24h). No reduction of viral titers is observed when treated with the 10X concentrated form or 0.1X diluted form of TNM after 24 h of contact.
Figure 10.
Loss of bacteriophage MS2 viability measured by the double-layer method. Representative plate images of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact. Plates of bacteriophages in contact with concentrated tiger nut milk (TNM 10X) for 2 h (TNM 10X 2h); concentrated TNM 10X for 5 h (TNM 10X 5h); concentrated TNM 10X for 15 h (TNM 10X 15h); concentrated TNM 10X for 24 h (TNM 10X 24h); diluted TNM 0.1X for 2 h (TNM 0.1X 2h); diluted TNM 0.1X for 5 h (TNM 0.1X 5h); diluted TNM 0.1X for 15 h (TNM 0.1X 15h) and diluted TNM 0.1X for 24 h (TNM 0.1X 24h). No reduction of viral titers is observed when treated with the 10X concentrated form or 0.1X diluted form of TNM after 24 h of contact.
Table 1.
Antiviral results of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) against the phi 6 bacteriophage after 2, 5, 15, and 24 h of contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
Table 1.
Antiviral results of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) against the phi 6 bacteriophage after 2, 5, 15, and 24 h of contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 4.96 × 106 ± 4.53 × 105 | - | - |
control BAK | 0.00 ± 0.00 | ~7 | 100 |
control dW | 3.11 × 106 ± 3.72 × 105 | ~0 | ~0 |
TNM 2h | 3.01 × 106 ± 6.52 × 105 | ~0 | ~0 |
TNM 5h | 3.21 × 106 ± 1.97 × 105 | ~0 | ~0 |
TNM 15h | 0.00 ± 0.00 | ~7 | 100 |
TNM 24h | 0.00 ± 0.00 | ~7 | 10 |
Table 2.
Antiviral effects of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) with 0.1 g/mL of sugar added against bacteriophage phi 6 after 2, 5, 15, and 24 h in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
Table 2.
Antiviral effects of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) with 0.1 g/mL of sugar added against bacteriophage phi 6 after 2, 5, 15, and 24 h in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 2.37 × 106 ± 5.33 × 105 | - | - |
control BAK | 0.00 ± 0.00 | ~6 | 100 |
control dW | 3.10 × 106 ± 8.77 × 105 | ~0 | ~0 |
TNM+S 2h | 1.57 × 106 ± 1.22 × 105 | ~0 | ~0 |
TNM+S 5h | 0.00 ± 0.00 | ~6 | 100 |
TNM+S 15h | 0.00 ± 0.00 | ~6 | 100 |
TNM+S 24h | 0.00 ± 0.00 | ~6 | 100 |
Table 3.
Antiviral results of concentrated tiger nut milk (TNM 10X) and diluted TNM 0.1X forms against bacteriophage phi 6 after different times (2, 5, 15, and 24 h) of viral contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
Table 3.
Antiviral results of concentrated tiger nut milk (TNM 10X) and diluted TNM 0.1X forms against bacteriophage phi 6 after different times (2, 5, 15, and 24 h) of viral contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 2.04 × 106 ± 2.83 × 105 | - | - |
control BAK | 0.00 ± 0.00 | ~6 | 100 |
control dW | 3.43 × 106 ± 1.84 × 105 | ~0 | ~0 |
TNM 10X 2h | 2.69 × 105 ± 7.26 × 104 | ~1 | ~87 |
TNM 10X 5h | 9.13 × 104 ± 8.72 × 104 | ~2 | ~96 |
TNM 10X 15h | 2.67 × 103 ± 3.06 × 103 | ~3 | ~100 |
TNM 10X 24h | 8.67 × 103 ± 6.11 × 103 | ~3 | ~100 |
TNM 0.1X 2h | 1.92 × 106 ± 2.88 × 105 | ~0 | ~0 |
TNM 0.1X 5h | 2.43 × 106 ± 1.67 × 105 | ~0 | ~0 |
TNM 0.1X 15h | 4.87 × 105 ± 1.30 × 105 | ~0.5 | ~76 |
TNM 0.1X 24h | 2.21 × 106 ± 3.03 × 105 | ~0 | ~0 |
Table 4.
Antiviral results of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) against MS2 bacteriophage after 2, 5, 15, and 24 h of viral contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) 24 h of viral contact.
Table 4.
Antiviral results of tiger nut milk (TNM) at a commercial concentration (0.32 g/mL) against MS2 bacteriophage after 2, 5, 15, and 24 h of viral contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 4.40 × 107 ± 5.66 × 106 | - | - |
control BAK | 0.00 ± 0.00 | ~8 | 100 |
control dW | 2.17 × 107 ± 6.23 × 106 | ~0 | ~0 |
TNM 2h | 1.31 × 107 ± 9.45 × 105 | ~0 | ~0 |
TNM 5h | 1.11 × 107 ± 1.21 × 106 | ~0 | ~0 |
TNM 15h | 9.27 × 106 ± 2.21 × 106 | ~0 | ~0 |
TNM 24h | 6.80 × 107 ± 6.93 × 106 | ~0 | ~0 |
Table 5.
Antiviral results of tiger nut milk (TNM) with 0.1 g/mL of sugar added against bacteriophage MS2 after 2, 5, 15, and 24 h of contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
Table 5.
Antiviral results of tiger nut milk (TNM) with 0.1 g/mL of sugar added against bacteriophage MS2 after 2, 5, 15, and 24 h of contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 1.54 × 108 ± 1.41 × 107 | - | - |
control BAK | 0.00 ± 0.00 | ~8 | 100 |
control dW | 2.17 × 108 ± 6.23 × 107 | ~0 | ~0 |
TNM+S 2h | 7.47 × 108 ± 6.11 × 107 | ~0 | ~0 |
TNM+S 5h | 1.14 × 108 ± 4.06 × 107 | ~0 | ~0 |
TNM+S 15h | 1.17 × 108 ± 1.92 × 107 | ~0 | ~0 |
TNM+S 24h | 3.23 × 107 ± 4.41 × 106 | ~1 | ~79 |
Table 6.
Antiviral results of concentrated tiger nut milk (TNM 10X) and diluted (TNM 0.1X) forms against bacteriophage MS2 after 2, 5, 15, and 24 h contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
Table 6.
Antiviral results of concentrated tiger nut milk (TNM 10X) and diluted (TNM 0.1X) forms against bacteriophage MS2 after 2, 5, 15, and 24 h contact in plaque-forming units per mL (PFU/mL). Representative values of bacteriophages in contact with tryptic soy broth (TSB) (control TSB), antiviral benzalkonium chloride (BAK) (control BAK), and distilled water (control dW) for 24 h of viral contact.
SAMPLE | VIRUS CONCENTRATION (PFU/mL) | Log10 REDUCTION | VIRAL INACTIVATION (%) |
---|
control TSB
| 2.64 × 107 ± 1.13 × 106 | - | - |
control BAK | 0.00 ± 0.00 | ~7 | 100 |
control dW | 5.64 × 107 ± 2.34 × 106 | ~0 | ~0 |
TNM 10X 2h | 1.44 × 107 ± 2.31 × 106 | ~0 | ~0 |
TNM 10X 5h | 8.47 × 106 ± 3.88 × 106 | ~0 | ~0 |
TNM 10X 15h | 1.15 × 107 ± 2.19 × 106 | ~0 | ~0 |
TNM 10X 24h | 8.85 × 107 ± 4.69 × 106 | ~0 | ~0 |
TNM 0.1X 2h | 1.36 × 107 ± 1.51 × 106 | ~0 | ~0 |
TNM 0.1X 5h | 3.18 × 107 ± 1.39 × 107 | ~0 | ~0 |
TNM 0.1X 15h | 1.05 × 107 ± 1.22 × 106 | ~0 | ~0 |
TNM 0.1X 24h | 8.13 × 107 ± 4.69 × 106 | ~0 | ~0 |