Comparative Analysis Based on Physiological and Transcriptomic Data between Juvenile and Adult Tree Peony (Paeonia delavayi)
Abstract
:1. Introduction
2. Results
2.1. Physiological Changes between Juvenile and Adult Plants
2.2. Differentially Expressed Genes (DEGs) between Juvenile and Adult Plants and Enriched Pathways of DEGs
2.2.1. Transcriptome Sequencing Assembly and Annotation
2.2.2. Screening of DEGs and Enrichment Analysis of DEGs
2.3. SPL Genes Expression Analysis
3. Discussion
3.1. Sugar Signaling (Possibly byT6P Pathway) May Mediate Juvenile-to-Adult Transition in P. delavayi
3.2. Hormone Signaling (Possibly in Interactive Way) May Mediate Juvenile-to-Adult Transition in P. delavayi
3.3. Circadian Rhythm Pathway May Mediate Juvenile-to-Adult Process in P. delavayi
3.4. Age Pathway May Mediate Juvenile-to-Adult Transition in P. delavayi
3.5. A Hypothetical Molecular Regulatory Network during Juvenile-to-Adult Transition in P. delavayi
4. Materials and Methods
4.1. Plant Materials
4.2. Methods
4.2.1. Quantitation of Carbohydrates and Plant Hormones
4.2.2. Discovery of DEGs and Pathways
4.2.3. Expression Analysis of SPL Genes
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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ID | Gene Annotation | Gene Name | J-FPKM | A-FPKM | Fold Change |
---|---|---|---|---|---|
c74517.graph_c0 | AP1/FUL-like protein | PdFUL | 5.55 | 32.18 | 5.80 |
c83958.graph_c0 | CONSTANS-LIKE 2 | PdCOL2 | 16.15 | 52.53 | 3.25 |
c85008.graph_c0 | FLOWERING-promoting factor 1 | PdFPF1 | 2.73 | 44.99 | 16.48 |
c89148.graph_c0 | protein LHY | PdLHY | 1.97 | 38.62 | 19.60 |
c71307.graph_c0 | promoter-binding protein SPL10 | PdSPL10 | 1.03 | 5.81 | 5.64 |
KEGG Pathway | ID | Gene Annotation | Up/Down-Regulated | Fold Change |
---|---|---|---|---|
Circadian rhythm-plant | c70450.graph_c0 | REVEILLE 1 | up | 4.12 |
c70837.graph_c0 | B-box zinc finger protein 19 | up | 4.49 | |
c83340.graph_c1 | Cyclic dof factor 3 | up | 5.07 | |
c83958.graph_c0 | CONSTANS-LIKE 2 | up | 3.25 | |
c84340.graph_c1 | REVEILLE 8 | up | 3.41 | |
c89148.graph_c0 | LHY | up | 19.57 | |
Plant hormone signal transduction | c41494.graph_c0 | Scarecrow-like protein 3 | up | 3.08 |
c53132.graph_c0 | Auxin-responsive protein SAUR32-like | up | 2.79 | |
c86094.graph_c1 | Auxin response factor 7 | up | 2.66 |
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Zhai, X.; Feng, Y.; Zhang, X.; Guo, X. Comparative Analysis Based on Physiological and Transcriptomic Data between Juvenile and Adult Tree Peony (Paeonia delavayi). Int. J. Mol. Sci. 2023, 24, 10906. https://doi.org/10.3390/ijms241310906
Zhai X, Feng Y, Zhang X, Guo X. Comparative Analysis Based on Physiological and Transcriptomic Data between Juvenile and Adult Tree Peony (Paeonia delavayi). International Journal of Molecular Sciences. 2023; 24(13):10906. https://doi.org/10.3390/ijms241310906
Chicago/Turabian StyleZhai, Xiaoli, Yan Feng, Xiuxin Zhang, and Xianfeng Guo. 2023. "Comparative Analysis Based on Physiological and Transcriptomic Data between Juvenile and Adult Tree Peony (Paeonia delavayi)" International Journal of Molecular Sciences 24, no. 13: 10906. https://doi.org/10.3390/ijms241310906