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Article

Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis

by
Yimian Ma
1,†,
Jiadong Ran
1,†,
Guoqiong Li
1,
Mengchen Wang
1,
Chengmin Yang
1,
Xin Wen
1,
Xin Geng
1,
Liping Zhang
1,
Yuan Li
2,* and
Zheng Zhang
1,*
1
National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
2
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(12), 9872; https://doi.org/10.3390/ijms24129872
Submission received: 6 May 2023 / Revised: 4 June 2023 / Accepted: 6 June 2023 / Published: 8 June 2023
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Jasmonate ZIM-domain family proteins (JAZs) are repressors in the signaling cascades triggered by jasmonates (JAs). It has been proposed that JAs play essential roles in the sesquiterpene induction and agarwood formation processes in Aquilaria sinensis. However, the specific roles of JAZs in A. sinensis remain elusive. This study employed various methods, including phylogenetic analysis, real-time quantitative PCR, transcriptomic sequencing, yeast two-hybrid assay, and pull-down assay, to characterize A. sinensis JAZ family members and explore their correlations with WRKY transcription factors. The bioinformatic analysis revealed twelve putative AsJAZ proteins in five groups and sixty-four putative AsWRKY transcription factors in three groups. The AsJAZ and AsWRKY genes exhibited various tissue-specific or hormone-induced expression patterns. Some AsJAZ and AsWRKY genes were highly expressed in agarwood or significantly induced by methyl jasmonate in suspension cells. Potential relationships were proposed between AsJAZ4 and several AsWRKY transcription factors. The interaction between AsJAZ4 and AsWRKY75n was confirmed by yeast two-hybrid and pull-down assays. This study characterized the JAZ family members in A. sinensis and proposed a model of the function of the AsJAZ4/WRKY75n complex. This will advance our understanding of the roles of the AsJAZ proteins and their regulatory pathways.
Keywords: jasmonate; sesquiterpene; JAZ protein; WRKY transcription factor; expression pattern; protein interaction; phylogenetic analysis jasmonate; sesquiterpene; JAZ protein; WRKY transcription factor; expression pattern; protein interaction; phylogenetic analysis

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MDPI and ACS Style

Ma, Y.; Ran, J.; Li, G.; Wang, M.; Yang, C.; Wen, X.; Geng, X.; Zhang, L.; Li, Y.; Zhang, Z. Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. Int. J. Mol. Sci. 2023, 24, 9872. https://doi.org/10.3390/ijms24129872

AMA Style

Ma Y, Ran J, Li G, Wang M, Yang C, Wen X, Geng X, Zhang L, Li Y, Zhang Z. Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. International Journal of Molecular Sciences. 2023; 24(12):9872. https://doi.org/10.3390/ijms24129872

Chicago/Turabian Style

Ma, Yimian, Jiadong Ran, Guoqiong Li, Mengchen Wang, Chengmin Yang, Xin Wen, Xin Geng, Liping Zhang, Yuan Li, and Zheng Zhang. 2023. "Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis" International Journal of Molecular Sciences 24, no. 12: 9872. https://doi.org/10.3390/ijms24129872

APA Style

Ma, Y., Ran, J., Li, G., Wang, M., Yang, C., Wen, X., Geng, X., Zhang, L., Li, Y., & Zhang, Z. (2023). Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process in Aquilaria sinensis. International Journal of Molecular Sciences, 24(12), 9872. https://doi.org/10.3390/ijms24129872

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