Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays
Abstract
:1. Introduction
2. Results
2.1. Morphological Analysis
2.2. Flow Cytometry Analysis
2.3. Evaluation of WJ-MSCs Differentiation
2.4. RNA-Seq Analysis
3. Discussion
4. Materials and Methods
4.1. Material Collection
4.2. Wharton’s Jelly-Derived Mesenchymal Stem Cells Isolation
4.3. In Vitro Cell Culture
4.4. Flow Cytometry Analysis
4.5. Multilineage Differentiation
4.5.1. Osteogenic Differentiation
4.5.2. Neurogenic Differentiation
4.5.3. Chondrogenic Differentiation
4.5.4. Adipogenic Differentiation
4.6. RNA Isolation
4.7. RNA-Seq
4.8. Bioinformatical and Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Stefańska, K.; Nemcova, L.; Blatkiewicz, M.; Pieńkowski, W.; Ruciński, M.; Zabel, M.; Mozdziak, P.; Podhorska-Okołów, M.; Dzięgiel, P.; Kempisty, B. Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays. Int. J. Mol. Sci. 2023, 24, 10023. https://doi.org/10.3390/ijms241210023
Stefańska K, Nemcova L, Blatkiewicz M, Pieńkowski W, Ruciński M, Zabel M, Mozdziak P, Podhorska-Okołów M, Dzięgiel P, Kempisty B. Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays. International Journal of Molecular Sciences. 2023; 24(12):10023. https://doi.org/10.3390/ijms241210023
Chicago/Turabian StyleStefańska, Katarzyna, Lucie Nemcova, Małgorzata Blatkiewicz, Wojciech Pieńkowski, Marcin Ruciński, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okołów, Piotr Dzięgiel, and Bartosz Kempisty. 2023. "Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays" International Journal of Molecular Sciences 24, no. 12: 10023. https://doi.org/10.3390/ijms241210023