Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit
Abstract
:1. Introduction
2. Results
2.1. Identification of FcNCED/CCDs Family Genes and Analysis of Deduced Amino Acid Sequences
2.2. Identification of FcPYR/PYL Family Genes and Analysis of Amino Acid Sequences
2.3. Expression Analysis of ABA Biosynthesis Related Genes in F. chiloensis
2.4. Expression Analysis of ABA Receptor Genes during Development of F. chiloensis Fruit and Vegetative Tissues
2.5. During Fruit Development ABA Levels Correlate with ABA Biosynthesis Genes and ABA Receptor Genes
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. F. chiloensis Gene Sequences
4.3. Phylogenetic Analysis and Motif Analysis
4.4. RNA Extraction and Expression Analysis by Real Time PCR (qPCR)
4.5. Heatmap Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Transcript ID | Domain Type | Seq Length (aa) |
---|---|---|---|
FcNCED1 | comp919_c0_seq1 | Retinal pigment epithelial membrane protein | 501 |
FcNCED3 | comp27198_c0_seq1 | Retinal pigment epithelial membrane protein | 392 |
FcCCD1 | comp799_c0_seq1 | Retinal pigment epithelial membrane protein | 568 |
FcCCD4 | comp503_c0_seq1 | Retinal pigment epithelial membrane protein | 581 |
Gene | Transcript ID | Domain Type | Seq Length (aa) |
---|---|---|---|
FcPYR1 | comp13086_c0_seq1 | Polyketide cyclase/dehydrase and lipid transport | 216 |
FcPYL2 | comp7632_c0_seq1 | ND | 93 |
FcPYL4 | comp22550_c0_seq1 | Polyketide cyclase/dehydrase and lipid transport | 218 |
FcPYL8 | comp2447_c0_seq3 | Polyketide cyclase/dehydrase and lipid transport | 189 |
FcPYL9 | comp3997_c0_seq2 | Polyketide cyclase/dehydrase and lipid transport | 121 |
FcPYL12 | comp2932_c0_seq1 | Polyketide cyclase/dehydrase and lipid transport | 167 |
Gene | Sequence(5′→3′) | Efficiency | |
---|---|---|---|
FcNCED1 | Fw | GATCTACCTTGGCGAAACCA | 90.0% |
Rv | GAGGCGGATCATGTGAACTT | ||
FcNCED3 | Fw | ACGACTTCGCCATTACCG | 92.1% |
Rv | AGCATCGCTCGATTCT | ||
FcCCD1 | Fw | GCCAAGCATATGACACTCCTC | 98.0% |
Rv | TCCTCGTTAGAAGGCCTGAA | ||
FcCCD4 | Fw | CATTCCCGACCAAGATAGGA | 94.3% |
Rv | GCCGTCCTTTGAGTAAACC | ||
FcPYL2 | Fw | GCCATGGTGGTCAACTGTTA | 100.5% |
Rv | CTGGGATTCTGGGGTACAC | ||
FcPYL4 | Fw | ATGCCTCCCAACCCACCCAA | 104.0% |
Rv | CGCTGCTGCTGCTGCTTCTT | ||
FcPYL8 | Fw | TGAAGCTTCCGAGCTTTCAT | 92.1% |
Rv | GGTCCTTAAACTTTGACGGAAG | ||
FcPYL9 | Fw | GATGCACCTGCTAACAAAAGG | 90.1% |
Rv | CAATCGTGTTTCTCATTTGTGC | ||
FcPYL11 | Fw | GAAAGGATGGCTGGTAATTGA | 91.0% |
Rv | CGCTACAACAAGAAGTCAAGAA | ||
FcPYL12 | Fw | TTTCCTATCCTGCTGCTGCT | 96.1% |
Rv | GCTTAGAATCCGAAACGGACTA | ||
FcPYR1 | Fw | GCTACCCAAATTGCTGAACC | 99.3% |
Rv | GAATGACGAAAATGTCCTTGG |
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Moya-León, M.A.; Stappung, Y.; Mattus-Araya, E.; Herrera, R. Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit. Int. J. Mol. Sci. 2023, 24, 8531. https://doi.org/10.3390/ijms24108531
Moya-León MA, Stappung Y, Mattus-Araya E, Herrera R. Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit. International Journal of Molecular Sciences. 2023; 24(10):8531. https://doi.org/10.3390/ijms24108531
Chicago/Turabian StyleMoya-León, María A., Yazmina Stappung, Elena Mattus-Araya, and Raúl Herrera. 2023. "Insights into the Genes Involved in ABA Biosynthesis and Perception during Development and Ripening of the Chilean Strawberry Fruit" International Journal of Molecular Sciences 24, no. 10: 8531. https://doi.org/10.3390/ijms24108531