Single-Cell RNA Sequencing Analysis for Oncogenic Mechanisms Underlying Oral Squamous Cell Carcinoma Carcinogenesis with Candida albicans Infection
Abstract
:1. Introduction
2. Results
2.1. Single-Cell RNA Sequencing in the Normal Tissue, OPMD Lesion with C. albicans Infection, OSCC Tissue with C. albicans Infection, and OSCC Tissue without C. albicans Infection
2.2. Cell Composition Change in the Normal Tissue, OPMD Lesion with C. albicans Infection, OSCC Tissue with C. albicans Infection, and OSCC Tissue without C. albicans Infection
2.3. Regulatory Pathways Involved in OSCC Carcinogenesis with and without C. albicans Infection
2.4. Stratifin (SFN) as the Specific Biomarker of OSCC with C. albicans Infection
3. Discussion
4. Materials and Methods
4.1. Clinical Patient Specimen Collection
4.2. Candidiasis Detection
4.3. Tissue Dissociation
4.4. 3′ Gene Expression Library Construction on 10× Genomics Platform
4.5. Next-Generation Sequencing
4.6. Bioinformatic Analysis of NGS Data
4.7. Immunohistochemistry Staining
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Hsieh, Y.-P.; Wu, Y.-H.; Cheng, S.-M.; Lin, F.-K.; Hwang, D.-Y.; Jiang, S.-S.; Chen, K.-C.; Chen, M.-Y.; Chiang, W.-F.; Liu, K.-J.; et al. Single-Cell RNA Sequencing Analysis for Oncogenic Mechanisms Underlying Oral Squamous Cell Carcinoma Carcinogenesis with Candida albicans Infection. Int. J. Mol. Sci. 2022, 23, 4833. https://doi.org/10.3390/ijms23094833
Hsieh Y-P, Wu Y-H, Cheng S-M, Lin F-K, Hwang D-Y, Jiang S-S, Chen K-C, Chen M-Y, Chiang W-F, Liu K-J, et al. Single-Cell RNA Sequencing Analysis for Oncogenic Mechanisms Underlying Oral Squamous Cell Carcinoma Carcinogenesis with Candida albicans Infection. International Journal of Molecular Sciences. 2022; 23(9):4833. https://doi.org/10.3390/ijms23094833
Chicago/Turabian StyleHsieh, Yi-Ping, Yu-Hsueh Wu, Siao-Muk Cheng, Fang-Kuei Lin, Daw-Yang Hwang, Shih-Sheng Jiang, Ken-Chung Chen, Meng-Yen Chen, Wei-Fan Chiang, Ko-Jiunn Liu, and et al. 2022. "Single-Cell RNA Sequencing Analysis for Oncogenic Mechanisms Underlying Oral Squamous Cell Carcinoma Carcinogenesis with Candida albicans Infection" International Journal of Molecular Sciences 23, no. 9: 4833. https://doi.org/10.3390/ijms23094833