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Article

Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice

by
Zhong Li
1,2,
Muhammad Umar Khan
2,
Xue Yan
1,
Dan Mu
1,
Yuebin Xie
1,
Muhammad Waqas
1,2,
Xin Wu
1,
Puleng Letuma
3,
Changxun Fang
1,2,* and
Wenxiong Lin
1,2,*
1
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Key Laboratory of Crop Ecology and Molecular Physiology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Crop Science Department, Faculty of Agriculture, National University of Lesotho, Maseru 100, Lesotho
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(9), 4667; https://doi.org/10.3390/ijms23094667
Submission received: 14 March 2022 / Revised: 19 April 2022 / Accepted: 21 April 2022 / Published: 23 April 2022
(This article belongs to the Special Issue Role of Silicon and Biochar in Plant Stress Tolerance)
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Abstract

Improving tolerance to low-temperature stress during the rice seedling stage is of great significance in agricultural science. In this study, using the low silicon gene 1 (Lsi1)-overexpressing (Dular-OE) and wild-type rice (Dular-WT), we showed that Lsi1 overexpression enhances chilling tolerance in Dular-OE. The overexpression of the Lsi1 increases silicon absorption, but it was not the main reason for chilling tolerance in Dular-OE. Instead, our data suggest that the overexpression of a Lsi1-encoding NIP and its interaction with key proteins lead to chilling tolerance in Dular-OE. Additionally, we show that the high-mobility group protein (HMG1) binds to the promoter of Lsi1, positively regulating its expression. Moreover, Nod26-like major intrinsic protein (NIP)’s interaction with α and β subunits of ATP synthase and the 14-3-3f protein was validated by co-immunoprecipitation (Co-IP), bimolecular fluorescent complementary (BiFC), and GST-pulldown assays. Western blotting revealed that the overexpression of NIP positively regulates the ATP-synthase subunits that subsequently upregulate calcineurin B-like interacting protein kinases (CIPK) negatively regulating 14-3-3f. Overall, these NIP-mediated changes trigger corresponding pathways in an orderly manner, enhancing chilling tolerance in Dular-OE.
Keywords: rice; silicon; low temperature sensitization; Lsi1 gene overexpression; proteomics; interacting proteins rice; silicon; low temperature sensitization; Lsi1 gene overexpression; proteomics; interacting proteins

Share and Cite

MDPI and ACS Style

Li, Z.; Umar Khan, M.; Yan, X.; Mu, D.; Xie, Y.; Waqas, M.; Wu, X.; Letuma, P.; Fang, C.; Lin, W. Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice. Int. J. Mol. Sci. 2022, 23, 4667. https://doi.org/10.3390/ijms23094667

AMA Style

Li Z, Umar Khan M, Yan X, Mu D, Xie Y, Waqas M, Wu X, Letuma P, Fang C, Lin W. Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice. International Journal of Molecular Sciences. 2022; 23(9):4667. https://doi.org/10.3390/ijms23094667

Chicago/Turabian Style

Li, Zhong, Muhammad Umar Khan, Xue Yan, Dan Mu, Yuebin Xie, Muhammad Waqas, Xin Wu, Puleng Letuma, Changxun Fang, and Wenxiong Lin. 2022. "Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice" International Journal of Molecular Sciences 23, no. 9: 4667. https://doi.org/10.3390/ijms23094667

APA Style

Li, Z., Umar Khan, M., Yan, X., Mu, D., Xie, Y., Waqas, M., Wu, X., Letuma, P., Fang, C., & Lin, W. (2022). Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice. International Journal of Molecular Sciences, 23(9), 4667. https://doi.org/10.3390/ijms23094667

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