Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Inhibitory Effect of MiRNA-3614-5p on Cell Viability in Human Prostate Cancer Cells
2.2. Inhibitory Effect of MiRNA-3614-5p on Colony Formation in Human Prostate Cancer Cells
2.3. Induction of Sub-G1 Cell Cycle Arrest by MiRNA-3614-5p in Human PC3 and 22Rv1 Prostate Cancer Cells
2.4. Induction of Apoptosis by MiRNA-3614-5p in Human PC3 and 22Rv1 Prostate Cancer Cells
2.5. MiRNA-3614-5p Is a Crucial Targeting on Mcl-1 in Human PC3 and 22Rv1 Prostate Cancer Cells
2.6. Induction of Pro-Apoptotic Caspase-3 and PARP Expressions by MiRNA-3614-5p in Human PC3 and 22Rv1 Prostate Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Chemical and Reagent
4.2. Cell Lines
4.3. Cell Proliferation Assay
4.4. Cell Cycle Distribution by Flow Cytometry
4.5. Colony Formation Assay
4.6. Annexin V/PI Staining by Flow Cytometry Analysis
4.7. Western Blotting Analysis
4.8. MiRNA Prediction
4.9. MiRNA Transient Transfection
4.10. Dual-Luciferase Reporter Assay
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hsieh, Y.-H.; Yu, F.-J.; Nassef, Y.; Liu, C.-J.; Chen, Y.-S.; Lin, C.-Y.; Feng, J.-L.; Wu, M.-H. Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells. Int. J. Mol. Sci. 2022, 23, 4194. https://doi.org/10.3390/ijms23084194
Hsieh Y-H, Yu F-J, Nassef Y, Liu C-J, Chen Y-S, Lin C-Y, Feng J-L, Wu M-H. Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells. International Journal of Molecular Sciences. 2022; 23(8):4194. https://doi.org/10.3390/ijms23084194
Chicago/Turabian StyleHsieh, Yi-Hsien, Fang-Jung Yu, Yasser Nassef, Chung-Jung Liu, Yong-Syuan Chen, Ching-Yi Lin, Jia-Liang Feng, and Min-Hua Wu. 2022. "Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells" International Journal of Molecular Sciences 23, no. 8: 4194. https://doi.org/10.3390/ijms23084194