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Article

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

1
Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, Poland
2
Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, Poland
3
Department of Neurosurgery, 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, Poland
4
Department of Neurosurgery and Neurology, Faculty of Health Sciences, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-168 Bydgoszcz, Poland
*
Authors to whom correspondence should be addressed.
Current address: Bioanalysis Scientific Group, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 85-089 Bydgoszcz, Poland.
Current address: Department of Neurosurgery and Neurology, Jan Biziel University Hospital Collegium Medicum, Nicolaus Copernicus University, 85-168 Bydgoszcz, Poland.
Academic Editor: Andreas Weigert
Int. J. Mol. Sci. 2022, 23(7), 3518; https://doi.org/10.3390/ijms23073518
Received: 26 February 2022 / Revised: 14 March 2022 / Accepted: 21 March 2022 / Published: 24 March 2022
(This article belongs to the Special Issue Gliomas: From Molecular Mechanisms to Therapeutic Approaches)
The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method of enabling the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology. View Full-Text
Keywords: brain tumor; glioma; SPME; heterogeneity; lipids; chemical biopsy brain tumor; glioma; SPME; heterogeneity; lipids; chemical biopsy
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MDPI and ACS Style

Bogusiewicz, J.; Kupcewicz, B.; Goryńska, P.Z.; Jaroch, K.; Goryński, K.; Birski, M.; Furtak, J.; Paczkowski, D.; Harat, M.; Bojko, B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. Int. J. Mol. Sci. 2022, 23, 3518. https://doi.org/10.3390/ijms23073518

AMA Style

Bogusiewicz J, Kupcewicz B, Goryńska PZ, Jaroch K, Goryński K, Birski M, Furtak J, Paczkowski D, Harat M, Bojko B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. International Journal of Molecular Sciences. 2022; 23(7):3518. https://doi.org/10.3390/ijms23073518

Chicago/Turabian Style

Bogusiewicz, Joanna, Bogumiła Kupcewicz, Paulina Zofia Goryńska, Karol Jaroch, Krzysztof Goryński, Marcin Birski, Jacek Furtak, Dariusz Paczkowski, Marek Harat, and Barbara Bojko. 2022. "Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes" International Journal of Molecular Sciences 23, no. 7: 3518. https://doi.org/10.3390/ijms23073518

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