CabZIP23 Integrates in CabZIP63–CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction
Abstract
:1. Introduction
2. Results
2.1. CabZIP23 Was Present in Proteins Isolated from CabZIP63-GFP Transiently Overexpressed in Pepper Leaves by Co-Immunoprecipitation Using an Antibody of GFP
2.2. CabZIP23 Is a Nuclear Protein and Has Transcriptional Activity
2.3. The Silencing of CabZIP23 Increased Susceptibility of Pepper Plants to RSI but Did Not Affect Pepper Thermotolerance
2.4. The Transient Overexpression of CabZIP23 Triggered Cell Death and Upregulated Immunity Related Marker Genes
2.5. CabZIP23 Targeted the Promoters of CaPR1 and CaNPR1 in a G-Box Dependent Manner but Not That of CaHSP24
2.6. CabZIP23 Was Targeted and Transcriptionally Regulated by CaWRKY40
2.7. CabZIP63 Was Directly Targeted and Transcriptionally Regulated by CabZIP23
2.8. The Confirmation of CabZIP23–CabZIP63 Interaction
2.9. CabZIP23 Promoted the Binding of CabZIP23 to the Promoters of CaPR1 and CaNPR1 as Well as CaWRKY40, thereby Promoting Their Activations by CabZIP63
3. Discussion
3.1. CabZIP23 Acts Positively in Pepper Response to RSI
3.2. CabZIP23 Integrates into the Feedback Loop between CaWRKY40 and CabZIP63
3.3. CabZIP23 Promotes CabZIP63 to Context Specifically Mount Immunity against RSI via Physical Interaction
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Pathogens and Inoculation Procedures
4.3. Vector Construction
4.4. VIGS
4.5. Transient Overexpression in Pepper Leaves
4.6. Subcellular Localization of CabZIP23
4.7. Yeast Transcriptional Activation
4.8. Measurement of Ion Conductivity
4.9. Chlorophyll Fluorescence Spectrophotometry
4.10. Histochemical Staining
4.11. ChIP Analysis
4.12. Prokaryotic Expression of Fusion Protein in Escherichia coli
4.13. Electrophoresis Mobility Shift Assay
4.14. Bimolecular Fluorescent Complimentary (BiFC) Assay
4.15. Co-Immunoprecipitation (Co-IP)
4.16. Quantitative Real-Time PCR (qRT-PCR) Assay
4.17. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lu, Q.; Huang, Y.; Wang, H.; Wan, M.; Lv, J.; Cheng, X.; Chen, Y.; Cai, W.; Yang, S.; Shen, L.; et al. CabZIP23 Integrates in CabZIP63–CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction. Int. J. Mol. Sci. 2022, 23, 2656. https://doi.org/10.3390/ijms23052656
Lu Q, Huang Y, Wang H, Wan M, Lv J, Cheng X, Chen Y, Cai W, Yang S, Shen L, et al. CabZIP23 Integrates in CabZIP63–CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction. International Journal of Molecular Sciences. 2022; 23(5):2656. https://doi.org/10.3390/ijms23052656
Chicago/Turabian StyleLu, Qiaoling, Yu Huang, Hui Wang, Meiyun Wan, Jingang Lv, Xingge Cheng, Yuanhui Chen, Weiwei Cai, Sheng Yang, Lei Shen, and et al. 2022. "CabZIP23 Integrates in CabZIP63–CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction" International Journal of Molecular Sciences 23, no. 5: 2656. https://doi.org/10.3390/ijms23052656