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Article

Overexpression of Liriodendron tulipifera JAG Gene (LtuJAG) Changes Leaf Shapes in Transgenic Arabidopsis thaliana

1
Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2
College of Forestry, Nanjing Forestry University, Nanjing 210037, China
*
Author to whom correspondence should be addressed.
Academic Editor: Pedro Martínez-Gómez
Int. J. Mol. Sci. 2022, 23(3), 1322; https://doi.org/10.3390/ijms23031322
Received: 21 December 2021 / Revised: 20 January 2022 / Accepted: 20 January 2022 / Published: 25 January 2022
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
In Arabidopsis thaliana, JAGGED (JAG) is a transcription inhibitor that controls the development of leaf polarity and regulates the expression of genes controlling lateral organ formation. Liriodendron tulipifera is an ornamental tree with extraordinary tulip-shaped flowers and goose web-like leaves, this is one of the suitable plants for morphological development research. To investigate the potential functions of the LtuJAG gene, we isolated the full-length LtuJAG from L. tulipifera, transferred it into A. thaliana via agrobacterium-mediated transformation, and monitored its expression pattern. Subcellular localization showed that LtuJAG was located in the nucleus. RT-qPCR assays indicated that LtuJAG was expressed mainly in leaf buds and flowers, but not in mature leaves and stems. GUS staining results showed that LtuJAG was expressed in the shoot apical meristem (SAM). Overexpressing LtuJAG changed A. thaliana leaf shapes, causing a moderate serration and a slight asymmetric distribution in the medio-lateral and proximal-distal axes. Ectopic expression of LtuJAG induced the expression of lateral organ boundary suppressors JAGGED LATERAL ORGANS (JLO) and ARABIDOPSIS THALIANA HOMEOBOX1 (ATH1). It also repressed the expression of the apical meristem suppressor class-1 KNOX gene (KNOX I) and altered endogenous hormone levels. Our results suggest that LtuJAG plays a role in negatively regulating leaf polarity formation in L. tulipifera. View Full-Text
Keywords: Liriodendron tulipifera; LtuJAG; leaf polarity; transformation; Arabidopsis thaliana Liriodendron tulipifera; LtuJAG; leaf polarity; transformation; Arabidopsis thaliana
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MDPI and ACS Style

Wei, L.; Wen, S.; Tu, Z.; Zhao, Y.; Li, H. Overexpression of Liriodendron tulipifera JAG Gene (LtuJAG) Changes Leaf Shapes in Transgenic Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 1322. https://doi.org/10.3390/ijms23031322

AMA Style

Wei L, Wen S, Tu Z, Zhao Y, Li H. Overexpression of Liriodendron tulipifera JAG Gene (LtuJAG) Changes Leaf Shapes in Transgenic Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(3):1322. https://doi.org/10.3390/ijms23031322

Chicago/Turabian Style

Wei, Lingmin, Shaoying Wen, Zhonghua Tu, Yanqing Zhao, and Huogen Li. 2022. "Overexpression of Liriodendron tulipifera JAG Gene (LtuJAG) Changes Leaf Shapes in Transgenic Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 3: 1322. https://doi.org/10.3390/ijms23031322

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