Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons
Abstract
:1. Introduction
2. Results
2.1. Different Expression of NCS-1 in the Cortical Cells in Culture In Vitro
2.2. Expression of NCS-1 Protects Neurons from OGD/Reoxygenation (OGD/R)-Induced Death through the Regulation of Apoptotic and Inflammatory Protein Expression
2.3. Expression of NCS-1 Protects Neurons from OGD/R-Induced Death through the Regulation of Calcium-Binding Protein Expression and Cytosolic Calcium Concentration
2.4. The Mechanism of the Neuroprotective Action of NCS-1 Occurs Due to the Suppression of Hyperexcitation of GABAergic Neurons and the Suppression of the Global [Ca2+]i Increase
3. Discussion
4. Materials and Methods
4.1. Preparation of Mixed Neuroglial Cell Cultures
4.2. Immunocytochemistry
4.3. Transfection with Small Interfering RNA (siRNA)
4.4. Fluorescent Ca2+ Measurements
4.5. The Technique for Simulation of Ischemia-like Conditions
4.6. Assessment of Cell Viability and Apoptosis
4.7. Extraction of RNA
4.8. Real-Time Polymerase Chain Reaction (RT-qPCR)
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Varlamova, E.G.; Plotnikov, E.Y.; Turovsky, E.A. Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons. Int. J. Mol. Sci. 2022, 23, 15675. https://doi.org/10.3390/ijms232415675
Varlamova EG, Plotnikov EY, Turovsky EA. Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons. International Journal of Molecular Sciences. 2022; 23(24):15675. https://doi.org/10.3390/ijms232415675
Chicago/Turabian StyleVarlamova, Elena G., Egor Y. Plotnikov, and Egor A. Turovsky. 2022. "Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons" International Journal of Molecular Sciences 23, no. 24: 15675. https://doi.org/10.3390/ijms232415675