Conservation Study of Imprinted Genes in Maize Triparental Heterozygotic Kernels
Abstract
:1. Introduction
2. Results
2.1. Analysis of Gene Expression and Identification of Imprinted Genes in Reciprocal Crosses
2.2. Conservation and GO Analysis of the Non-Conserved Genes
2.3. Cis-Element Distribution, Expression Pattern and Subcellular Location of Proteins from the Conserved and Non-Conserved Imprinted Genes
2.4. Extremely Non-Conserved Imprinted Genes Detected in Different Species
2.5. Phenotype Analysis of the Non-Conserved PEG Zm00001d020769 Transgenic Line
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Library Construction for RNA-Seq
4.3. Read Mapping, Gene Expression Analysis and SNP Calling
4.4. Measuring Allelic Expression and Identification of Imprinted Genes
4.5. GO Term Enrichment and Functional Category Analysis
4.6. Validation of Imprinted Genes
4.7. Genetic Transformation of Maize
4.8. Primers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Dong, X.; Luo, H.; Yao, J.; Guo, Q.; Yu, S.; Zhang, X.; Li, F.; Ruan, Y.; Jin, W.; Meng, D. Conservation Study of Imprinted Genes in Maize Triparental Heterozygotic Kernels. Int. J. Mol. Sci. 2022, 23, 15424. https://doi.org/10.3390/ijms232315424
Dong X, Luo H, Yao J, Guo Q, Yu S, Zhang X, Li F, Ruan Y, Jin W, Meng D. Conservation Study of Imprinted Genes in Maize Triparental Heterozygotic Kernels. International Journal of Molecular Sciences. 2022; 23(23):15424. https://doi.org/10.3390/ijms232315424
Chicago/Turabian StyleDong, Xiaomei, Haishan Luo, Jiabin Yao, Qingfeng Guo, Shuai Yu, Xiaoyu Zhang, Fenghai Li, Yanye Ruan, Weiwei Jin, and Dexuan Meng. 2022. "Conservation Study of Imprinted Genes in Maize Triparental Heterozygotic Kernels" International Journal of Molecular Sciences 23, no. 23: 15424. https://doi.org/10.3390/ijms232315424