Unveiling the Metal-Dependent Aggregation Properties of the C-terminal Region of Amyloidogenic Intrinsically Disordered Protein Isoforms DPF3b and DPF3a
Abstract
:1. Introduction
2. Results
2.1. DPF3 C-terminal Regions Have Intrinsic Disorder Properties
2.2. DPF3 C-terminal Regions Are Prone to Aggregate In Vitro
2.3. Transmission Electron Microscopy Reveals the Morphologic Diversity of DPF3 C-terminal Region Aggregates
2.4. Metal Cations Have Distinct Effects on the Aggregation of DPF3 C-terminal Regions
3. Discussion
4. Materials and Methods
4.1. Overexpression and Purification of C-terminal Regions
4.2. Protein Concentration and UV–Visible Absorption Spectroscopy
4.3. Metal Cation and Protein Sample Preparation
4.4. Bioinformatics Analyses
4.5. Far- and Near-UV Circular Dichroism Spectroscopy
4.6. Intrinsic Fluorescence Spectroscopy (ITF, ITyrF, and dbAF)
4.7. Thioflavin T Binding Assay
4.8. Transmission Electron Microscopy
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Secondary Structure Content (%) | ||||||
---|---|---|---|---|---|---|
C-TER (time) | RMSD | α-Helix | Antiparallel β-Sheet | Parallel β-Sheet | Turn | Coil |
C-TERb (0 h) | 0.04 | 4 | 38 | 0 | 17 | 40 |
C-TERb (24 h) | 0.05 | 5 | 35 | 0 | 15 | 46 |
C-TERb (48 h) | 0.05 | 0 | 38 | 3 | 16 | 43 |
C-TERb (72 h) | 0.08 | 0 | 24 | 9 | 21 | 46 |
C-TERb (96 h) | 0.08 | 0 | 24 | 15 | 21 | 40 |
C-TERa (0 h) | 0.03 | 2 | 32 | 0 | 18 | 48 |
C-TERa (24 h) | 0.02 | 0 | 37 | 0 | 18 | 45 |
C-TERa (48 h) | 0.04 | 0 | 37 | 0 | 16 | 47 |
C-TERa (72 h) | 0.06 | 0 | 34 | 5 | 19 | 42 |
C-TERa (96 h) | 0.07 | 0 | 25 | 10 | 22 | 43 |
Secondary Structure Content (%) | ||||||
---|---|---|---|---|---|---|
C-TER (M2+) | RMSD | α-Helix | Antiparallel β-Sheet | Parallel β-Sheet | Turn | Coil |
C-TERb | 0.08 | 0 | 24 | 9 | 21 | 46 |
C-TERb (Cu2+) | 0.06 | 0 | 31 | 8 | 18 | 43 |
C-TERb (Mg2+) | 0.06 | 0 | 27 | 8 | 20 | 45 |
C-TERb (Ni2+) | 0.07 | 0 | 33 | 6 | 20 | 41 |
C-TERb (Zn2+) | 0.07 | 0 | 30 | 7 | 19 | 44 |
C-TERa | 0.06 | 0 | 34 | 5 | 19 | 42 |
C-TERa (Cu2+) | 0.05 | 0 | 33 | 5 | 17 | 45 |
C-TERa (Mg2+) | 0.09 | 0 | 21 | 11 | 23 | 45 |
C-TERa (Ni2+) | 0.05 | 0 | 38 | 3 | 18 | 41 |
C-TERa (Zn2+) | 0.06 | 0 | 38 | 3 | 18 | 41 |
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Leyder, T.; Mignon, J.; Mottet, D.; Michaux, C. Unveiling the Metal-Dependent Aggregation Properties of the C-terminal Region of Amyloidogenic Intrinsically Disordered Protein Isoforms DPF3b and DPF3a. Int. J. Mol. Sci. 2022, 23, 15291. https://doi.org/10.3390/ijms232315291
Leyder T, Mignon J, Mottet D, Michaux C. Unveiling the Metal-Dependent Aggregation Properties of the C-terminal Region of Amyloidogenic Intrinsically Disordered Protein Isoforms DPF3b and DPF3a. International Journal of Molecular Sciences. 2022; 23(23):15291. https://doi.org/10.3390/ijms232315291
Chicago/Turabian StyleLeyder, Tanguy, Julien Mignon, Denis Mottet, and Catherine Michaux. 2022. "Unveiling the Metal-Dependent Aggregation Properties of the C-terminal Region of Amyloidogenic Intrinsically Disordered Protein Isoforms DPF3b and DPF3a" International Journal of Molecular Sciences 23, no. 23: 15291. https://doi.org/10.3390/ijms232315291