Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress
Abstract
:1. Introduction
2. Results
2.1. Acute Exposure to HMW-AGEs Alters the Vasomotor Function
2.2. Preincubation with Superoxide Dismutase Improves Endothelium-Dependent Relaxation after Acute HMW-Ages Exposure
3. Discussion
3.1. Acute Exposure to HMW-AGEs Impairs Endothelium-Dependent Relaxation
3.2. The Impaired Endothelium-Dependent Relaxation Is Caused by Increased Superoxide Formation
3.3. Acute HMW-AGEs Exposure Tends to Increase Contractile Vascular Reactivity
4. Materials and Methods
4.1. Animal Experiments
4.2. Preparation and Protein Concentration Determination of HMW-AGEs
4.3. Assessment of Aortic Vasomotor Function
4.3.1. General Procedure
4.3.2. Vasorelaxation and Vasocontraction Responses
4.4. Immunohistochemistry
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Groups | Emax (%) | LogEC50 (M) | |
---|---|---|---|
ACh | Non-exposed | 85.09 ± 1.95 | −6.81 ± 0.07 |
HMW-AGEs | 71.18 ± 2.98 * | −6.54 ± 0.07 * | |
Non-exposed + SOD | 81.24 ± 4.04 | −7.01 ± 0.10 | |
HMW-AGEs + SOD | 93.89 ± 1.50 × | −7.41 ± 0.07 × | |
SNP | Non-exposed | 99.71 ± 0.05 | −8.39 ± 0.05 |
HMW-AGEs | 99.23 ± 0.07 | −8.25 ± 0.05 | |
PE | Non-exposed | 3.14 ± 0.14 | −7.14 ± 0.06 |
HMW-AGEs | 3.29 ± 0.23 | −7.35 ± 0.11 * |
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D’Haese, S.; Deluyker, D.; Bito, V. Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress. Int. J. Mol. Sci. 2022, 23, 14916. https://doi.org/10.3390/ijms232314916
D’Haese S, Deluyker D, Bito V. Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress. International Journal of Molecular Sciences. 2022; 23(23):14916. https://doi.org/10.3390/ijms232314916
Chicago/Turabian StyleD’Haese, Sarah, Dorien Deluyker, and Virginie Bito. 2022. "Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress" International Journal of Molecular Sciences 23, no. 23: 14916. https://doi.org/10.3390/ijms232314916