Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors
Abstract
:1. Introduction
2. Results
2.1. Pomt2 Mutation in Zebrafish Caused Diminished Expression of Matriglycan and EYS Binding
2.2. Pomt2 Mutant Zebrafish Exhibited Hydrocephalus, Hypoplasia of the Cerebellum, and Muscular Dystrophy
2.3. EYS Protein Was Reduced and Mislocalized in the Pomt2 Mutant Retina
2.4. Pomt2 Mutant Zebrafish Exhibited Photoreceptor Degeneration
3. Discussion
4. Materials and Methods
4.1. Zebrafish Maintenance
4.2. Generation of the Pomt2sny5+13 Mutant Line
4.3. RT-PCR
4.4. Western Blotting Overlay Experiment
4.5. Immunofluorescence Staining
4.6. Semi-Quantitative Analyses Immunofluorescence Staining Signals
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Age of Animals | +/+ | +/− | −/− | p Value * |
---|---|---|---|---|
7-dpf | 23 | 20 | 12 | 0.59 |
14-dpf | 14 | 26 | 15 | 0.70 |
21-dpf | 20 | 21 | 15 | 0.76 |
1-mpf | 45 | 83 | 30 | 0.081 |
2-mpf | 28 | 62 | 14 | 0.0066 |
3–6-mpf | 169 | 264 | 38 | 2.13 × 10−17 |
Age Range | +/+ | +/− | −/− | p Value * |
---|---|---|---|---|
21 to 35 dpf | 2 | 17 | 12 | 0.034 |
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Liu, Y.; Rittershaus, J.M.; Yu, M.; Sager, R.; Hu, H. Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors. Int. J. Mol. Sci. 2022, 23, 14809. https://doi.org/10.3390/ijms232314809
Liu Y, Rittershaus JM, Yu M, Sager R, Hu H. Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors. International Journal of Molecular Sciences. 2022; 23(23):14809. https://doi.org/10.3390/ijms232314809
Chicago/Turabian StyleLiu, Yu, Jaclyn M. Rittershaus, Miao Yu, Rachel Sager, and Huaiyu Hu. 2022. "Deletion of POMT2 in Zebrafish Causes Degeneration of Photoreceptors" International Journal of Molecular Sciences 23, no. 23: 14809. https://doi.org/10.3390/ijms232314809