The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance
Abstract
:1. Introduction
2. Results
2.1. Changes in Laccase Activity and Lignin Content in Response to Cold Stress in C. sinensis
2.2. Expression Analysis of CsLACs in Response to Cold Treatment
2.3. Cloning, Sequence Analysis, and Subcellular Localization of CsLAC18
2.4. Overexpression of CsLAC18 Confers Enhanced Cold Tolerance
2.5. Silencing of CsLAC18 in Poncirus Trifoliata increases Cold Sensitivity
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Cold Treatment
4.2. Determination of Laccase Activity and Lignin Content
4.3. RNA Extraction and Quantitative Real-Time PCR Analysis
4.4. Cloning and Sequence Analysis of CsLAC18
4.5. Subcellular Localization Analysis of CsLAC18
4.6. Vector Construction and Plant Transformation
4.7. Virus-Induced Gene Silencing
4.8. Cold Tolerance Assays, Physiological Measurements and Histochemical Staining
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, X.; Zhang, Y.; Liang, M.; Kong, W.; Liu, J. The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance. Int. J. Mol. Sci. 2022, 23, 14509. https://doi.org/10.3390/ijms232314509
Xu X, Zhang Y, Liang M, Kong W, Liu J. The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance. International Journal of Molecular Sciences. 2022; 23(23):14509. https://doi.org/10.3390/ijms232314509
Chicago/Turabian StyleXu, Xiaoyong, Yueliang Zhang, Mengge Liang, Weiwen Kong, and Jihong Liu. 2022. "The Citrus Laccase Gene CsLAC18 Contributes to Cold Tolerance" International Journal of Molecular Sciences 23, no. 23: 14509. https://doi.org/10.3390/ijms232314509