Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Antiviral Activity
2.2. Body Weight and Temperature
2.3. Fluoxetine Modulates the Ceramide System
2.4. Anti-Viral Activity of Fluoxetine In Vitro against Variants of Concern
2.5. Anti-Viral Activity of Fluoxetine Ex Vivo on Human Primary Airway Epithelial Cells
3. Discussion
4. Materials and Methods
4.1. Safety Procedures
4.2. SARS-CoV-2 Virus Production
4.3. Virus Titration by TCID50 Calculation
4.4. Cell Infection and Treatment with Fluoxetine
4.5. Air Liquid Interface (ALI) Cultures
4.6. Cell Death and Viability
4.7. Pharmacokinetics of Fluoxetine in Mice
4.8. Mouse Infection
4.9. Quantitative Real-Time PCR Analysis of Viral RNA and Inflammatory Genes
4.10. Sphingolipid Quantification by Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS)
4.11. Dual A549 Cell Stimulation
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Péricat, D.; Leon-Icaza, S.A.; Sanchez Rico, M.; Mühle, C.; Zoicas, I.; Schumacher, F.; Planès, R.; Mazars, R.; Gros, G.; Carpinteiro, A.; et al. Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model. Int. J. Mol. Sci. 2022, 23, 13623. https://doi.org/10.3390/ijms232113623
Péricat D, Leon-Icaza SA, Sanchez Rico M, Mühle C, Zoicas I, Schumacher F, Planès R, Mazars R, Gros G, Carpinteiro A, et al. Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model. International Journal of Molecular Sciences. 2022; 23(21):13623. https://doi.org/10.3390/ijms232113623
Chicago/Turabian StylePéricat, David, Stephen Adonai Leon-Icaza, Marina Sanchez Rico, Christiane Mühle, Iulia Zoicas, Fabian Schumacher, Rémi Planès, Raoul Mazars, Germain Gros, Alexander Carpinteiro, and et al. 2022. "Antiviral and Anti-Inflammatory Activities of Fluoxetine in a SARS-CoV-2 Infection Mouse Model" International Journal of Molecular Sciences 23, no. 21: 13623. https://doi.org/10.3390/ijms232113623