Changes in α-Farnesene and Phenolic Metabolism and the Expression of Associated Genes during the Development of Superficial Scald in Two Distinct Pear Cultivars
Abstract
:1. Introduction
2. Results
2.1. Changes in the Superficial Scald Index
2.2. Changes in the Levels of α-Farnesene and CTols and Their Related Gene Expression
2.3. Changes in the Phenolic Content and Their Related Gene Expression
2.4. Changes in PPO and LAC Gene Expression
2.5. Changes in Cell Membrane Permeability
2.6. PCA Analysis
3. Discussion
3.1. CTols Have a Great Influence on the Occurrence of Scald Compared to α-Farnesene
3.2. Phenols and Their Oxidation Reactions
4. Materials and Methods
4.1. Plant Materials
4.2. Experimental Design
4.3. Measurement of the Superficial Scald Index
4.4. Determination of α-Farnesene and CTols Contents
4.5. Estimation of the Phenolic Component Contents
4.6. Relative Conductivity Measurement
4.7. RNA Isolation and Real-Time PCR (RT-PCR) Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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He, J.; Feng, Y.; Cheng, Y.; Karuppanapandian, T.; Wang, J.; Guan, J. Changes in α-Farnesene and Phenolic Metabolism and the Expression of Associated Genes during the Development of Superficial Scald in Two Distinct Pear Cultivars. Int. J. Mol. Sci. 2022, 23, 12088. https://doi.org/10.3390/ijms232012088
He J, Feng Y, Cheng Y, Karuppanapandian T, Wang J, Guan J. Changes in α-Farnesene and Phenolic Metabolism and the Expression of Associated Genes during the Development of Superficial Scald in Two Distinct Pear Cultivars. International Journal of Molecular Sciences. 2022; 23(20):12088. https://doi.org/10.3390/ijms232012088
Chicago/Turabian StyleHe, Jingang, Yunxiao Feng, Yudou Cheng, Thirupathi Karuppanapandian, Jinxiao Wang, and Junfeng Guan. 2022. "Changes in α-Farnesene and Phenolic Metabolism and the Expression of Associated Genes during the Development of Superficial Scald in Two Distinct Pear Cultivars" International Journal of Molecular Sciences 23, no. 20: 12088. https://doi.org/10.3390/ijms232012088