The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice
Abstract
:1. Introduction
2. Results
2.1. Expression Pattern of OsWRKY54
2.2. Subcellular Localization of OsWRKY54
2.3. Cell-Specific Expression of OsWRKY54
2.4. Knockout of OsWRKY54 Decreased Salt Tolerance of Rice
2.5. Loss of OsWRKY54 Alters Shoot Na+/K+ Homeostasis of Transgenic Rice
2.6. Transcriptional Activation Analysis of OsWRKY54
2.7. OsWRKY54 Affects the Expression Profiles of Many Stress-Related Genes
2.8. OsHKT1;5 Is a Direct Target of OsWRKY54
3. Discussion
4. Materials and Methods
4.1. Generation of OsWRKY54 Knockout Mutants
4.2. Plant Materials and Growth Conditions
4.3. Gene Expression Analysis
4.4. Subcellular Localization of OsWRKY54
4.5. Tissue Localization of OsWRKY54
4.6. Physiological Characterization of OsWRKY54 Mutant Lines
4.7. Na/K Concentration in Xylem Sap
4.8. Determination of Na/K Concentration
4.9. RNA-Seq Assay
4.10. Dual-LUC Transient Expression Assay
4.11. Yeast Assays
4.12. EMSA
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Huang, J.; Liu, F.; Chao, D.; Xin, B.; Liu, K.; Cao, S.; Chen, X.; Peng, L.; Zhang, B.; Fu, S.; et al. The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice. Int. J. Mol. Sci. 2022, 23, 11999. https://doi.org/10.3390/ijms231911999
Huang J, Liu F, Chao D, Xin B, Liu K, Cao S, Chen X, Peng L, Zhang B, Fu S, et al. The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice. International Journal of Molecular Sciences. 2022; 23(19):11999. https://doi.org/10.3390/ijms231911999
Chicago/Turabian StyleHuang, Jingjing, Fuhang Liu, Dong Chao, Boning Xin, Kui Liu, Shuling Cao, Xingxiang Chen, Liyun Peng, Baolei Zhang, Shan Fu, and et al. 2022. "The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice" International Journal of Molecular Sciences 23, no. 19: 11999. https://doi.org/10.3390/ijms231911999
APA StyleHuang, J., Liu, F., Chao, D., Xin, B., Liu, K., Cao, S., Chen, X., Peng, L., Zhang, B., Fu, S., & Xia, J. (2022). The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice. International Journal of Molecular Sciences, 23(19), 11999. https://doi.org/10.3390/ijms231911999