Targeting the Mitochondrial Protein VDAC1 as a Potential Therapeutic Strategy in ALS
Abstract
:1. Introduction
2. Results
2.1. Identification of the VDAC1 Interaction Site in SOD1
2.2. VBIT-12 Rescued Cell Death Induced by Mutant SOD1 in NSC-34 Motor-Neuron-like Cells
2.3. VDAC1 Oligomerization Is Increased in Mutant SOD1G93A Spinal Cord Mitochondria
2.4. VDAC1 Expression Levels Are Increased in Mutant SOD1G93A Spinal Motor Neurons
2.5. VBIT-12 Administration Significantly Improved Muscle endurance in Mutant SOD1G93A Mice
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Synthetic Peptides
4.3. Peptide Arrays
4.4. VDAC1-Based Peptide Interaction Analysis Using Microscale Thermophoresis (MST)
4.5. Cell Culture
4.6. Crosslinking Experiments
4.7. Purified Proteins
4.8. Gel Electrophoresis and Immunoblotting
4.9. Animals and VBIT-4 or VBIT-12 Treatment
4.10. Immunofluorescence (IF) Staining
4.11. Immunoprecipitation
4.12. Quantification and Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shteinfer-Kuzmine, A.; Argueti-Ostrovsky, S.; Leyton-Jaimes, M.F.; Anand, U.; Abu-Hamad, S.; Zalk, R.; Shoshan-Barmatz, V.; Israelson, A. Targeting the Mitochondrial Protein VDAC1 as a Potential Therapeutic Strategy in ALS. Int. J. Mol. Sci. 2022, 23, 9946. https://doi.org/10.3390/ijms23179946
Shteinfer-Kuzmine A, Argueti-Ostrovsky S, Leyton-Jaimes MF, Anand U, Abu-Hamad S, Zalk R, Shoshan-Barmatz V, Israelson A. Targeting the Mitochondrial Protein VDAC1 as a Potential Therapeutic Strategy in ALS. International Journal of Molecular Sciences. 2022; 23(17):9946. https://doi.org/10.3390/ijms23179946
Chicago/Turabian StyleShteinfer-Kuzmine, Anna, Shirel Argueti-Ostrovsky, Marcel F. Leyton-Jaimes, Uttpal Anand, Salah Abu-Hamad, Ran Zalk, Varda Shoshan-Barmatz, and Adrian Israelson. 2022. "Targeting the Mitochondrial Protein VDAC1 as a Potential Therapeutic Strategy in ALS" International Journal of Molecular Sciences 23, no. 17: 9946. https://doi.org/10.3390/ijms23179946