GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton
Abstract
:1. Introduction
2. Results
2.1. GhBOP1 Bioinformatics and Expression Analysis
2.2. Interaction of GhBOP1 with GhPES and GhWDR12
2.3. Subcellular Localization of GhBOP1 and GhPES/GhWDR12
2.4. Phenotype of VIGS in GhBOP1 in Cotton
2.5. Expression of Jasmonic Acid, Cell Cycle, and Auxin Related Genes in GhBOP1-Silenced Lines
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Bioinformatics Analysis
4.3. Yeast Two-Hybrid Assays
4.4. Split Luciferase Complementation Assay (Split-LUC)
4.5. Bimolecular Fluorescence Complementation (BiFC)
4.6. Subcellular Localization of GhBOP1 and GhWDR12/GhPES
4.7. Virus-Induced Gene Silencing of BOP1 in Cotton and Paraffin Section of Leaf
4.8. Real-Time Quantitative PCR Detecting
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Wang, Y.; Sun, Z.; Wang, L.; Chen, L.; Ma, L.; Lv, J.; Qiao, K.; Fan, S.; Ma, Q. GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton. Int. J. Mol. Sci. 2022, 23, 9942. https://doi.org/10.3390/ijms23179942
Wang Y, Sun Z, Wang L, Chen L, Ma L, Lv J, Qiao K, Fan S, Ma Q. GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton. International Journal of Molecular Sciences. 2022; 23(17):9942. https://doi.org/10.3390/ijms23179942
Chicago/Turabian StyleWang, Yanwen, Zhimao Sun, Long Wang, Lingling Chen, Lina Ma, Jiaoyan Lv, Kaikai Qiao, Shuli Fan, and Qifeng Ma. 2022. "GhBOP1 as a Key Factor of Ribosomal Biogenesis: Development of Wrinkled Leaves in Upland Cotton" International Journal of Molecular Sciences 23, no. 17: 9942. https://doi.org/10.3390/ijms23179942