The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification
Abstract
:1. Introduction
2. Results
2.1. Screening GWAS
2.2. KIF26B Is Expressed in Human Bone, in BMP2 Stimulated Human Mesenchymal Stem Cells, Co-Localises with Microtubules in the Cytoskeleton, and Is Induced in a Mammalian Post-traumatic HO Model
2.3. Knockout of Murine Kif26b Prevents BMP2-Mediated Mineralisation of C2C12 Myoblasts
2.4. Kif26b Knockout Disrupts the Mineralisation and Trans-Differentiation of C2C12 Myoblasts by Modulating the Expression of Osteogenic Genes
2.5. KIF26B Deficiency Inhibits ERK MAP Kinase Activation during Osteogenesis, Whilst Augmenting p38 and SMAD 1/5/8 Phosphorylation
3. Discussion
4. Materials and Methods
4.1. Study Oversight and Populations
4.2. Screening GWAS
4.3. Cell Culture
4.4. CRISPR-Cas9 Knockout
4.5. Osteogenic Differentiation
4.6. RNA Isolation and RT-qPCR
4.7. Protein Isolation and Western Blotting
4.8. Alizarin Red S Staining
4.9. Immunocytochemistry
4.10. Immunohistochemistry
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pickering, G.A.E.; Felix-Ilemhenbhio, F.; Clark, M.J.; Kocsy, K.; Simpson, J.; Bellantuono, I.; Gartland, A.; Wilkinson, J.M.; Hatzikotoulas, K.; Kiss-Toth, E. The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification. Int. J. Mol. Sci. 2022, 23, 9203. https://doi.org/10.3390/ijms23169203
Pickering GAE, Felix-Ilemhenbhio F, Clark MJ, Kocsy K, Simpson J, Bellantuono I, Gartland A, Wilkinson JM, Hatzikotoulas K, Kiss-Toth E. The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification. International Journal of Molecular Sciences. 2022; 23(16):9203. https://doi.org/10.3390/ijms23169203
Chicago/Turabian StylePickering, George A. E., Favour Felix-Ilemhenbhio, Matthew J. Clark, Klaudia Kocsy, Jonathan Simpson, Ilaria Bellantuono, Alison Gartland, Jeremy Mark Wilkinson, Konstantinos Hatzikotoulas, and Endre Kiss-Toth. 2022. "The Kinesin Gene KIF26B Modulates the Severity of Post-Traumatic Heterotopic Ossification" International Journal of Molecular Sciences 23, no. 16: 9203. https://doi.org/10.3390/ijms23169203