Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites
Abstract
:1. Introduction
2. Results
2.1. Meg8-DMR Acts as an Insulator Dependent on the CTCF Binding Sites
2.2. Deletion of Meg8-DMR Reduced the Expression of Many Genes on Chromosome 12
2.3. Meg8-DMR Deletion Enhanced Migration and Invasion of MLTC-1
2.4. Overexpression of Dlk1 in Meg8-DMR-KO Suppressed Cells’ Migration and Invasion by Blocking Notch1-Rhoc-MAPK/ERK
2.5. Absence of Meg8-DMR Has No Impact on the Methylation of Other DMRs in the Dlk1-Dio3 Domain
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. The Enhancer Blocking Assay
4.3. CRISPR/Cas9-Mediated Deletion of Meg8-DMR, CTCF Binding Sites and GGCG Repeats
4.4. DNA Extraction and Methylation Analysis
4.5. RNA Extraction and RNA-Seq Analysis
4.6. qRT-PCR and Strand-Specific RT-PCR
4.7. Immunofluorescence
4.8. Wound Healing, Cell Migration and Invasion
4.9. MTT and Colony Formation Assays
4.10. Western Blotting Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Han, X.; He, H.; Shao, L.; Cui, S.; Yu, H.; Zhang, X.; Wu, Q. Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites. Int. J. Mol. Sci. 2022, 23, 8828. https://doi.org/10.3390/ijms23158828
Han X, He H, Shao L, Cui S, Yu H, Zhang X, Wu Q. Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites. International Journal of Molecular Sciences. 2022; 23(15):8828. https://doi.org/10.3390/ijms23158828
Chicago/Turabian StyleHan, Xiao, Hongjuan He, Lan Shao, Shuang Cui, Haoran Yu, Ximeijia Zhang, and Qiong Wu. 2022. "Deletion of Meg8-DMR Enhances Migration and Invasion of MLTC-1 Depending on the CTCF Binding Sites" International Journal of Molecular Sciences 23, no. 15: 8828. https://doi.org/10.3390/ijms23158828