Sanguinarine Inhibition of TNF-α-Induced CCL2, IKBKE/NF-κB/ERK1/2 Signaling Pathway, and Cell Migration in Human Triple-Negative Breast Cancer Cells

Round 1

Reviewer 1 Report

Dear author

 

Thank you for the submission of your article to our journal. I enjoyed your paper except for one point as follow;

 

The sentence ”SANG inhibited cell migration compared to MDA-MB-468 cells. ” is strange.

Author Response

 

Thank you for the submission of your article to our journal. I enjoyed your paper except for one point as follows;

The sentence ”SANG inhibited cell migration compared to MDA-MB-468 cells. ” is strange.

Response:

Thank you so much for this remarkable observation. The authors appreciate that, and we indeed agree with the reviewer. The authors revised as follows and highlighted in the manuscript.

SANG exhibited a higher potential to inhibit cell migration in MDA-MB-231 cells compared to MDA-MB-468 cells

 

 

Reviewer 2 Report

The manuscript entitled: “Sanguinarine Inhibition of TNF-α-Induced CCL2, IKBKE/NF-ƙB/ERK1/2 Signaling Pathway, and Cell Migration in Human Triple-Negative Breast Cancer Cells” reports a study on possible application of natural compounds, in particular the natural alkaloid sanguinarine, as a promising option for managing breast cancer. The experimenal assessment is properly made and the study t is interesting. It fits scope and aims of the Journal. A few minor remarks are reported in the following.

The end points, the overall experimental plan and the limits of the proposed study should be clearly stated and commented in the text. The readability of the manuscript should be improved, it is not easy to follow when reading.

The origin of the human cell lines used in the proposed study should clearly stated as well as all the necessary ethical committee authorization for the study. In vivo studies should be needed to corroborate the Authors Conclusions, albeit interesting. This aspect should bettter expoloited in the text as well as the limits and novelty of the proposed study. The mechanism of action regarding the antiangiogenic potential of the natural alkaloid compound sanguinarine should be better expolited in the text.

Too dated Refrences (e.g. see the one dated 1989, etc.) should be avoided in favor of more recent available published data or justifed in the text for their use.

Author Response

The authors would like to thank the reviewer for the constructive comments and suggestions. Please find the attached file.

Author Response File: Author Response.pdf