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Article

The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection

1
College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China
2
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
3
Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China
4
Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangzhou 510640, China
*
Author to whom correspondence should be addressed.
Academic Editor: Pedro Martinez-Gomez
Int. J. Mol. Sci. 2022, 23(13), 6971; https://doi.org/10.3390/ijms23136971
Received: 5 May 2022 / Revised: 20 June 2022 / Accepted: 20 June 2022 / Published: 23 June 2022
(This article belongs to the Special Issue Advances in Research for Fruit Crop Breeding and Genetics)
The citrus industry has been threatened by Huanglongbing (HLB) for over a century. Here, an HLB-induced Arabidopsis RPM1-interacting protein 4 (RIN4) homologous gene was cloned from Citrus clementina, and its characteristics and function were analyzed to determine its role during citrus–Candidatus Liberibacter asiaticus (CLas) interactions. Quantitative real-time PCR showed that RIN4 was expressed in roots, stems, leaves and flowers, with the greatest expression level in leaves. Its expression was suppressed by gibberellic acid, indole-3-acetic acid, salicylic acid and jasmonic acid treatments, but was induced by abscisic acid and salt treatments, as well as wounding. The transient expression of a RIN4-GFP showed that RIN4 was localized in the cell membrane. RIN4-overexpressing transgenic C. maxima cv. ‘Shatianyou’ plants were obtained, and some transgenic plants showed greater sensitivity to CLas infection and earlier HLB symptoms appearance than non-transgenic controls. Results obtained in this study indicated that the upregulated expression of RIN4 in HLB diseased citrus may aid CLas infection. View Full-Text
Keywords: citrus; RIN4; Huanglongbing; disease response; genetic transformation citrus; RIN4; Huanglongbing; disease response; genetic transformation
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MDPI and ACS Style

Cheng, C.; Zhong, Y.; Wang, B.; Zhang, Y.; Wu, H.; Jiang, N.; Wu, B.; Lv, Y.; Jiang, B. The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection. Int. J. Mol. Sci. 2022, 23, 6971. https://doi.org/10.3390/ijms23136971

AMA Style

Cheng C, Zhong Y, Wang B, Zhang Y, Wu H, Jiang N, Wu B, Lv Y, Jiang B. The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection. International Journal of Molecular Sciences. 2022; 23(13):6971. https://doi.org/10.3390/ijms23136971

Chicago/Turabian Style

Cheng, Chunzhen, Yun Zhong, Bin Wang, Yongyan Zhang, Huan Wu, Nonghui Jiang, Bo Wu, Yuanda Lv, and Bo Jiang. 2022. "The Upregulated Expression of the Citrus RIN4 Gene in HLB Diseased Citrus Aids Candidatus Liberibacter Asiaticus Infection" International Journal of Molecular Sciences 23, no. 13: 6971. https://doi.org/10.3390/ijms23136971

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