Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta)
Abstract
:1. Introduction
2. Results
2.1. Effect of Maturity Stage at Harvest on Changes in Phenotypic Characterization
2.2. The Effect of Maturity Stage at Harvest on Changes in the Nutritional Quality of the Fruit
2.3. Comparison of ASA and DHA Content
2.4. Activities of Key Enzymes Involved in AsA Metabolism
2.5. DEGs Obtained and qRT-PCR Validation
2.6. Expression Patterns of AsA Metabolism Genes
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Sample Preparation
4.2. Determination of Fruit Quality
4.3. HPLC Analysis of AsA
4.4. Measurement of AsA Metabolism–Related Enzymes
4.5. RNA-seq and Selection of Genes Involved in the AsA Matabolism
4.6. qRT-PCR Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lei, D.; Lin, Y.; Chen, Q.; Zhao, B.; Tang, H.; Zhang, Y.; Chen, Q.; Wang, Y.; Li, M.; He, W.; et al. Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta). Int. J. Mol. Sci. 2022, 23, 6816. https://doi.org/10.3390/ijms23126816
Lei D, Lin Y, Chen Q, Zhao B, Tang H, Zhang Y, Chen Q, Wang Y, Li M, He W, et al. Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta). International Journal of Molecular Sciences. 2022; 23(12):6816. https://doi.org/10.3390/ijms23126816
Chicago/Turabian StyleLei, Diya, Yuanxiu Lin, Qiyang Chen, Bing Zhao, Honglan Tang, Yunting Zhang, Qing Chen, Yan Wang, Mengyao Li, Wen He, and et al. 2022. "Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta)" International Journal of Molecular Sciences 23, no. 12: 6816. https://doi.org/10.3390/ijms23126816