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Article

Crosstalk between the Arabidopsis Glutathione Peroxidase-Like 5 Isoenzyme (AtGPXL5) and Ethylene

1
Department of Plant Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52., H-6726 Szeged, Hungary
2
Biological Research Centre, Institute of Plant Biology, Temesvári krt. 62., H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Academic Editors: Jen-Tsung Chen and Parviz Heidari
Int. J. Mol. Sci. 2022, 23(10), 5749; https://doi.org/10.3390/ijms23105749
Received: 8 February 2022 / Revised: 16 May 2022 / Accepted: 17 May 2022 / Published: 20 May 2022
(This article belongs to the Special Issue Cell Signaling in Model Plants 2.0)
Glutathione peroxidases (GPXs) are important antioxidant enzymes in animals. Plants contain GPX-like (GPXL) enzymes, which—in contrast to GPXs—contain cysteine in their active site instead of selenocysteine. Although several studies proved their importance in development and stress responses, their interaction with ethylene (ET) signalling is not known. Our aim was to investigate the involvement of AtGPXL5 in ET biosynthesis and/or signalling using Atgpxl5 mutant and AtGPXL5 cDNA-overexpressing (OX-AtGPXL5) lines. Four-day-old dark-grown Atgpxl5 seedlings had shorter hypocotyls and primary roots, while OX-AtGPXL5 seedlings exhibited a similar phenotype as wild type under normal conditions. Six-week-old OX-AtGPXL5 plants contained less H2O2 and malondialdehyde, but higher polyamine and similar ascorbate- and glutathione contents and redox potential (EGSH) than the Col-0. One-day treatment with the ET-precursor 1-aminocyclopropane-1-carboxylic acid (ACC) induced the activity of glutathione- and thioredoxin peroxidases and some other ROS-processing enzymes. In the Atgpxl5 mutants, the EGSH became more oxidised; parallelly, it produced more ethylene after the ACC treatment than other genotypes. Although the enhanced ET evolution measured in the Atgpxl5 mutant can be the result of the increased ROS level, the altered expression pattern of ET-related genes both in the Atgpxl5 and OX-AtGPXL5 plants suggests the interplay between AtGPXL5 and ethylene signalling. View Full-Text
Keywords: antioxidant mechanism; Arabidopsis thaliana; ethylene; glutathione peroxidase-like 5 enzyme; reactive oxygen species antioxidant mechanism; Arabidopsis thaliana; ethylene; glutathione peroxidase-like 5 enzyme; reactive oxygen species
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MDPI and ACS Style

Riyazuddin, R.; Bela, K.; Poór, P.; Szepesi, Á.; Horváth, E.; Rigó, G.; Szabados, L.; Fehér, A.; Csiszár, J. Crosstalk between the Arabidopsis Glutathione Peroxidase-Like 5 Isoenzyme (AtGPXL5) and Ethylene. Int. J. Mol. Sci. 2022, 23, 5749. https://doi.org/10.3390/ijms23105749

AMA Style

Riyazuddin R, Bela K, Poór P, Szepesi Á, Horváth E, Rigó G, Szabados L, Fehér A, Csiszár J. Crosstalk between the Arabidopsis Glutathione Peroxidase-Like 5 Isoenzyme (AtGPXL5) and Ethylene. International Journal of Molecular Sciences. 2022; 23(10):5749. https://doi.org/10.3390/ijms23105749

Chicago/Turabian Style

Riyazuddin, Riyazuddin, Krisztina Bela, Péter Poór, Ágnes Szepesi, Edit Horváth, Gábor Rigó, László Szabados, Attila Fehér, and Jolán Csiszár. 2022. "Crosstalk between the Arabidopsis Glutathione Peroxidase-Like 5 Isoenzyme (AtGPXL5) and Ethylene" International Journal of Molecular Sciences 23, no. 10: 5749. https://doi.org/10.3390/ijms23105749

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