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Article

Genome-Wide Identification and Expression Analyses of the Aquaporin Gene Family in Passion Fruit (Passiflora edulis), Revealing PeTIP3-2 to Be Involved in Drought Stress

by
Shun Song
1,2,
Dahui Zhang
3,
Funing Ma
1,2,
Wenting Xing
1,
Dongmei Huang
1,
Bin Wu
1,
Jian Chen
3,
Di Chen
1,
Binqiang Xu
1 and
Yi Xu
1,2,*
1
Key Laboratory of Genetic Improvement of Bananas, Haikou Experimental Station/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
Hainan Yazhou Bay Seed Laboratory, Sanya 571101, China
3
Yunnan Agricultural University, Kunming 650201, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(10), 5720; https://doi.org/10.3390/ijms23105720
Submission received: 1 April 2022 / Revised: 10 May 2022 / Accepted: 16 May 2022 / Published: 20 May 2022
(This article belongs to the Special Issue Regulatory Mechanism and Network of Abiotic Stress-Response in Plants)
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Abstract

Aquaporins (AQPs) in plants can transport water and small molecules, and they play an important role in plant development and abiotic stress response. However, to date, a comprehensive study on AQP family members is lacking. In this study, 27 AQP genes were identified from the passion fruit genome and classified into four groups (NIP, PIP, TIP, SIP) on the basis of their phylogenetic relationships. The prediction of protein interactions indicated that the AQPs of passion fruit were mainly associated with AQP family members and boron protein family genes. Promoter cis-acting elements showed that most PeAQPs contain light response elements, hormone response elements, and abiotic stress response elements. According to collinear analysis, passion fruit is more closely related to Arabidopsis than rice. Furthermore, three different fruit ripening stages and different tissues were analyzed on the basis of the transcriptome sequencing results for passion fruit AQPs under drought, high-salt, cold and high-temperature stress, and the results were confirmed by qRT-PCR. The results showed that the PeAQPs were able to respond to different abiotic stresses, and some members could be induced by and expressed in response to multiple abiotic stresses at the same time. Among the three different fruit ripening stages, 15 AQPs had the highest expression levels in the first stage. AQPs are expressed in all tissues of the passion fruit. One of the passion fruit aquaporin genes, PeTIP3-2, which was induced by drought stress, was selected and transformed into Arabidopsis. The survival rate of transgenic plants under drought stress treatment is higher than that of wild-type plants. The results indicated that PeTIP3-2 was able to improve the drought resistance of plants. Our discovery lays the foundation for the functional study of AQPs in passion fruit.
Keywords: aquaporin (AQP); abiotic stress; passion fruit; expression analysis; fruit maturity stage aquaporin (AQP); abiotic stress; passion fruit; expression analysis; fruit maturity stage

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MDPI and ACS Style

Song, S.; Zhang, D.; Ma, F.; Xing, W.; Huang, D.; Wu, B.; Chen, J.; Chen, D.; Xu, B.; Xu, Y. Genome-Wide Identification and Expression Analyses of the Aquaporin Gene Family in Passion Fruit (Passiflora edulis), Revealing PeTIP3-2 to Be Involved in Drought Stress. Int. J. Mol. Sci. 2022, 23, 5720. https://doi.org/10.3390/ijms23105720

AMA Style

Song S, Zhang D, Ma F, Xing W, Huang D, Wu B, Chen J, Chen D, Xu B, Xu Y. Genome-Wide Identification and Expression Analyses of the Aquaporin Gene Family in Passion Fruit (Passiflora edulis), Revealing PeTIP3-2 to Be Involved in Drought Stress. International Journal of Molecular Sciences. 2022; 23(10):5720. https://doi.org/10.3390/ijms23105720

Chicago/Turabian Style

Song, Shun, Dahui Zhang, Funing Ma, Wenting Xing, Dongmei Huang, Bin Wu, Jian Chen, Di Chen, Binqiang Xu, and Yi Xu. 2022. "Genome-Wide Identification and Expression Analyses of the Aquaporin Gene Family in Passion Fruit (Passiflora edulis), Revealing PeTIP3-2 to Be Involved in Drought Stress" International Journal of Molecular Sciences 23, no. 10: 5720. https://doi.org/10.3390/ijms23105720

APA Style

Song, S., Zhang, D., Ma, F., Xing, W., Huang, D., Wu, B., Chen, J., Chen, D., Xu, B., & Xu, Y. (2022). Genome-Wide Identification and Expression Analyses of the Aquaporin Gene Family in Passion Fruit (Passiflora edulis), Revealing PeTIP3-2 to Be Involved in Drought Stress. International Journal of Molecular Sciences, 23(10), 5720. https://doi.org/10.3390/ijms23105720

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