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Article

Transient Focal Cerebral Ischemia Leads to miRNA Alterations in Different Brain Regions, Blood Serum, Liver, and Spleen

by
Clara Voelz
1,
Nahal Ebrahimy
1,
Weiyi Zhao
1,
Pardes Habib
2,3,
Adib Zendedel
1,
Thomas Pufe
4,
Cordian Beyer
1 and
Alexander Slowik
4,*
1
Institute of Neuroanatomy, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany
2
Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany
3
JARA-BRAIN Institute of Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH, RWTH Aachen University, 52074 Aachen, Germany
4
Department of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(1), 161; https://doi.org/10.3390/ijms23010161
Submission received: 29 September 2021 / Revised: 17 December 2021 / Accepted: 21 December 2021 / Published: 23 December 2021
(This article belongs to the Special Issue Molecular Signaling in Stroke)
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Abstract

Ischemic stroke is characterized by an occlusion of a cerebral blood vessel resulting in neuronal cell death due to nutritional and oxygen deficiency. Additionally, post-ischemic cell death is augmented after reperfusion. These events are paralleled by dysregulated miRNA expression profiles in the peri-infarct area. Understanding the underlying molecular mechanism in the peri-infarct region is crucial for developing promising therapeutics. Utilizing a tMCAo (transient Middle Cerebral Artery occlusion) model in rats, we studied the expression levels of the miRNAs (miR) 223-3p, 155-5p, 3473, and 448-5p in the cortex, amygdala, thalamus, and hippocampus of both the ipsi- and contralateral hemispheres. Additionally, the levels in the blood serum, spleen, and liver and the expression of their target genes, namely, Nlrp3, Socs1, Socs3, and Vegfa, were assessed. We observed an increase in all miRNAs on the ipsilateral side of the cerebral cortex in a time-dependent manner and increased miRNAs levels (miR-223-3p, miR-3473, and miR-448-5p) in the contralateral hemisphere after 72 h. Besides the cerebral cortex, the amygdala presented increased expression levels, whereas the thalamus and hippocampus showed no alterations. Different levels of the investigated miRNAs were detected in blood serum, liver, and spleen. The gene targets were altered not only in the peri-infarct area of the cortex but selectively increased in the investigated non-affected brain regions along with the spleen and liver during the reperfusion time up to 72 h. Our results suggest a supra-regional influence of miRNAs following ischemic stroke, which should be studied to further identify whether miRNAs are transported or locally upregulated.
Keywords: tMCAo; miRNA regulation; CNS; organ crosstalk; intercellular transfer tMCAo; miRNA regulation; CNS; organ crosstalk; intercellular transfer

Share and Cite

MDPI and ACS Style

Voelz, C.; Ebrahimy, N.; Zhao, W.; Habib, P.; Zendedel, A.; Pufe, T.; Beyer, C.; Slowik, A. Transient Focal Cerebral Ischemia Leads to miRNA Alterations in Different Brain Regions, Blood Serum, Liver, and Spleen. Int. J. Mol. Sci. 2022, 23, 161. https://doi.org/10.3390/ijms23010161

AMA Style

Voelz C, Ebrahimy N, Zhao W, Habib P, Zendedel A, Pufe T, Beyer C, Slowik A. Transient Focal Cerebral Ischemia Leads to miRNA Alterations in Different Brain Regions, Blood Serum, Liver, and Spleen. International Journal of Molecular Sciences. 2022; 23(1):161. https://doi.org/10.3390/ijms23010161

Chicago/Turabian Style

Voelz, Clara, Nahal Ebrahimy, Weiyi Zhao, Pardes Habib, Adib Zendedel, Thomas Pufe, Cordian Beyer, and Alexander Slowik. 2022. "Transient Focal Cerebral Ischemia Leads to miRNA Alterations in Different Brain Regions, Blood Serum, Liver, and Spleen" International Journal of Molecular Sciences 23, no. 1: 161. https://doi.org/10.3390/ijms23010161

APA Style

Voelz, C., Ebrahimy, N., Zhao, W., Habib, P., Zendedel, A., Pufe, T., Beyer, C., & Slowik, A. (2022). Transient Focal Cerebral Ischemia Leads to miRNA Alterations in Different Brain Regions, Blood Serum, Liver, and Spleen. International Journal of Molecular Sciences, 23(1), 161. https://doi.org/10.3390/ijms23010161

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