Functional Analysis of Autophagy-Related Gene ATG12 in Potato Dry Rot Fungus Fusarium oxysporum
Abstract
:1. Introduction
2. Results
2.1. Gene Replacement of OEFoATG12 and ∆ATG12
2.2. Impaired Fungal Development Exhibited in the ∆ATG12 Mutants
2.3. Response against Stress
2.4. Plant Infection Assay
2.5. Expression of Pathogenicity and Vegetative Growth Related Genes
3. Discussion
4. Materials and Methods
4.1. Isolation of the Fungal Strain and the Culture Conditions
4.2. Fungal Transformation
4.3. Generation of FoATG12 Deletion Mutants
4.4. Overexpression (OE) of the Mutant FoATG12 Strains
4.5. Evaluation of Fungal Germination, Conidia Formation, and Radial Growth
4.6. Evaluation of the Fungal Sensitivity against Stress
4.7. Analysis of Gene Expression
4.8. Microscopic Analysis
4.9. Pathogenicity Test
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Khalid, A.R.; Zhang, S.; Luo, X.; Shaheen, H.; Majeed, A.; Maqbool, M.; Zahid, N.; Rahim, J.; Ren, M.; Qiu, D. Functional Analysis of Autophagy-Related Gene ATG12 in Potato Dry Rot Fungus Fusarium oxysporum. Int. J. Mol. Sci. 2021, 22, 4932. https://doi.org/10.3390/ijms22094932
Khalid AR, Zhang S, Luo X, Shaheen H, Majeed A, Maqbool M, Zahid N, Rahim J, Ren M, Qiu D. Functional Analysis of Autophagy-Related Gene ATG12 in Potato Dry Rot Fungus Fusarium oxysporum. International Journal of Molecular Sciences. 2021; 22(9):4932. https://doi.org/10.3390/ijms22094932
Chicago/Turabian StyleKhalid, A. Rehman, Shumin Zhang, Xiumei Luo, Hamayun Shaheen, Afshan Majeed, Mehdi Maqbool, Noosheen Zahid, Junaid Rahim, Maozhi Ren, and Dan Qiu. 2021. "Functional Analysis of Autophagy-Related Gene ATG12 in Potato Dry Rot Fungus Fusarium oxysporum" International Journal of Molecular Sciences 22, no. 9: 4932. https://doi.org/10.3390/ijms22094932