Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach
Abstract
:1. Introduction
2. Results
2.1. Comparison between Whole Saliva and Salivary EVs Protein Profiles
2.2. Identification of a Meta-Module of Co-Expressed Proteins Related to an Inflammatory Phenotype of Sjögren’s Syndrome
2.3. Differential Expression Protein Analysis Reveals the Relationship between Sjogren’s Syndrome and Innate Immune Response
2.4. Immunometric Assessment of Specific Inflammatory Proteins in Sjogren’s Syndrome
3. Discussion
4. Material and Methods
4.1. Patients
4.2. Saliva Collection
4.3. Salivary EVs Purification and Sample Preparation for Mass Spectrometry-Based Proteomics
4.4. Dynamic Light Scattering Analysis
4.5. MS Acquisitions: IDA and SWATH-MS
4.6. Spectral Library Generation and Statistical Analysis
4.7. Protein Co-Expression Network Analysis and Protein–Protein Interaction Network Construction
4.8. Functional Network Generation and Gene Ontology (GO) Terms Enrichment
4.9. Data Availability
4.10. Western Blot Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Finamore, F.; Cecchettini, A.; Ceccherini, E.; Signore, G.; Ferro, F.; Rocchiccioli, S.; Baldini, C. Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach. Int. J. Mol. Sci. 2021, 22, 4864. https://doi.org/10.3390/ijms22094864
Finamore F, Cecchettini A, Ceccherini E, Signore G, Ferro F, Rocchiccioli S, Baldini C. Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach. International Journal of Molecular Sciences. 2021; 22(9):4864. https://doi.org/10.3390/ijms22094864
Chicago/Turabian StyleFinamore, Francesco, Antonella Cecchettini, Elisa Ceccherini, Giovanni Signore, Francesco Ferro, Silvia Rocchiccioli, and Chiara Baldini. 2021. "Characterization of Extracellular Vesicle Cargo in Sjögren’s Syndrome through a SWATH-MS Proteomics Approach" International Journal of Molecular Sciences 22, no. 9: 4864. https://doi.org/10.3390/ijms22094864