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Volume 22
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Article

Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro

by
Piotr Chmielarz
1,†,
Justyna Kuśmierczyk
1,†,
Katarzyna Rafa-Zabłocka
1,
Katarzyna Chorązka
1,
Marta Kowalska
1,
Grzegorz Satała
2 and
Irena Nalepa
1,*
1
Department of Brain Biochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland
2
Department of Medicinal Chemistry, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this study.
Int. J. Mol. Sci. 2021, 22(9), 4817; https://doi.org/10.3390/ijms22094817
Submission received: 17 March 2021 / Revised: 28 April 2021 / Accepted: 29 April 2021 / Published: 1 May 2021
(This article belongs to the Collection Feature Papers in Molecular Neurobiology)
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Abstract

Currently utilized antidepressants have limited effectiveness and frequently incur undesired effects. Most antidepressants are thought to act via the inhibition of monoamine reuptake; however, direct binding to monoaminergic receptors has been proposed to contribute to both their clinical effectiveness and their side effects, or lack thereof. Among the target receptors of antidepressants, α1‑adrenergic receptors (ARs) have been implicated in depression etiology, antidepressant action, and side effects. However, differences in the direct effects of antidepressants on signaling from the three subtypes of α1-ARs, namely, α1A-, α1B- and α1D‑ARs, have been little explored. We utilized cell lines overexpressing α1A-, α1B- or α1D-ARs to investigate the effects of the antidepressants imipramine (IMI), desipramine (DMI), mianserin (MIA), reboxetine (REB), citalopram (CIT) and fluoxetine (FLU) on noradrenaline-induced second messenger generation by those receptors. We found similar orders of inhibition at α1A-AR (IMI < DMI < CIT < MIA < REB) and α1D‑AR (IMI = DMI < CIT < MIA), while the α1B-AR subtype was the least engaged subtype and was inhibited with low potency by three drugs (MIA < IMI = DMI). In contrast to their direct antagonistic effects, prolonged incubation with IMI and DMI increased the maximal response of the α1B-AR subtype, and the CIT of both the α1A- and the α1B-ARs. Our data demonstrate a complex, subtype-specific modulation of α1-ARs by antidepressants of different groups.
Keywords: alpha1-adrenergic receptor subtypes; antidepressants; imipramine; desipramine; mianserin; citalopram; antagonist; second messenger; inositol phosphate; G-protein-coupled receptor alpha1-adrenergic receptor subtypes; antidepressants; imipramine; desipramine; mianserin; citalopram; antagonist; second messenger; inositol phosphate; G-protein-coupled receptor

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MDPI and ACS Style

Chmielarz, P.; Kuśmierczyk, J.; Rafa-Zabłocka, K.; Chorązka, K.; Kowalska, M.; Satała, G.; Nalepa, I. Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro. Int. J. Mol. Sci. 2021, 22, 4817. https://doi.org/10.3390/ijms22094817

AMA Style

Chmielarz P, Kuśmierczyk J, Rafa-Zabłocka K, Chorązka K, Kowalska M, Satała G, Nalepa I. Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro. International Journal of Molecular Sciences. 2021; 22(9):4817. https://doi.org/10.3390/ijms22094817

Chicago/Turabian Style

Chmielarz, Piotr, Justyna Kuśmierczyk, Katarzyna Rafa-Zabłocka, Katarzyna Chorązka, Marta Kowalska, Grzegorz Satała, and Irena Nalepa. 2021. "Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro" International Journal of Molecular Sciences 22, no. 9: 4817. https://doi.org/10.3390/ijms22094817

APA Style

Chmielarz, P., Kuśmierczyk, J., Rafa-Zabłocka, K., Chorązka, K., Kowalska, M., Satała, G., & Nalepa, I. (2021). Antidepressants Differentially Regulate Intracellular Signaling from α1-Adrenergic Receptor Subtypes In Vitro. International Journal of Molecular Sciences, 22(9), 4817. https://doi.org/10.3390/ijms22094817

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