Effect of Whole Tissue Culture and Basic Fibroblast Growth Factor on Maintenance of Tie2 Molecule Expression in Human Nucleus Pulposus Cells
Abstract
:1. Introduction
2. Results
2.1. The Effect of WTC Method and FGF2 on Tie2 Expression Rates
2.2. Tie2 Expression and Signal Transduction Pathway by cFGF
2.3. Effects of MEK, Akt Inhibitors on FGF2 Signaling and Tie2 Expression
2.4. Correlation Between Tie2 Expression by FGF2 and COL2A1 Expression
3. Discussion
4. Materials and Methods
4.1. Human NP Cell Isolation
4.2. WTC Method
4.3. PCC Method
4.4. Assessment of FGF2 on Tie2 Expression in WTC and PCC
4.5. Role PI3K/Akt and MEK/ERK in FGF2 and cFGF Supported Tie2 Maintenance
4.6. Flow Cytometry and Fluorescence-Activated Cell Sorting
4.7. Western Blotting
4.8. Real-Time Polymerase Chain Reaction Analysis
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Code | Age (Year) | Sex | Pathology | IVD Tissue (g) | NP Tissue (g) |
---|---|---|---|---|---|
E32 | 32 | F | LDH | 4.75 | 4.75 |
A24 | 24 | M | LDH | 3.65 | 3.30 |
A20 | 20 | M | LDH | 6.98 | 3.23 |
T20 | 20 | M | LDH | 1.89 | 1.25 |
T21 | 21 | F | LDH | 2.82 | 1.81 |
A25 | 25 | F | LDH | 2.07 | 1.92 |
T19 | 19 | M | LDH | 8.05 | 7.59 |
A18 | 18 | M | LDH | 5.88 | 3.73 |
A14 | 14 | F | LDH | 1.74 | 1.54 |
A18-2 | 18 | M | LDH | 1.74 | 1.42 |
A29 | 29 | M | LDH | 2.68 | 1.77 |
T18 | 18 | M | LDH | 3.07 | 2.70 |
A18-3 | 18 | M | LDH | 2.16 | 1.26 |
A21 | 21 | M | LDH | 1.75 | 1.36 |
A20-2 | 20 | F | LDH | 1.06 | 0.98 |
A16 | 16 | M | LDH | 1.81 | 1.50 |
T16 | 16 | M | LDH | 2.03 | 2.03 |
K30F | 30 | M | LDH | 3.18 | 1.03 |
K30M | 30 | F | LDH | 4.26 | 2.5 |
K21 | 21 | M | LDH | 1.79 | 0.85 |
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Sako, K.; Sakai, D.; Nakamura, Y.; Schol, J.; Matsushita, E.; Warita, T.; Horikita, N.; Sato, M.; Watanabe, M. Effect of Whole Tissue Culture and Basic Fibroblast Growth Factor on Maintenance of Tie2 Molecule Expression in Human Nucleus Pulposus Cells. Int. J. Mol. Sci. 2021, 22, 4723. https://doi.org/10.3390/ijms22094723
Sako K, Sakai D, Nakamura Y, Schol J, Matsushita E, Warita T, Horikita N, Sato M, Watanabe M. Effect of Whole Tissue Culture and Basic Fibroblast Growth Factor on Maintenance of Tie2 Molecule Expression in Human Nucleus Pulposus Cells. International Journal of Molecular Sciences. 2021; 22(9):4723. https://doi.org/10.3390/ijms22094723
Chicago/Turabian StyleSako, Kosuke, Daisuke Sakai, Yoshihiko Nakamura, Jordy Schol, Erika Matsushita, Takayuki Warita, Natsumi Horikita, Masato Sato, and Masahiko Watanabe. 2021. "Effect of Whole Tissue Culture and Basic Fibroblast Growth Factor on Maintenance of Tie2 Molecule Expression in Human Nucleus Pulposus Cells" International Journal of Molecular Sciences 22, no. 9: 4723. https://doi.org/10.3390/ijms22094723