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Article

HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages

1
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
2
Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan
3
Department of Pathology, School of Medicine, Tokai University, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan
4
Department of Genomic Function and Diversity, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
5
Department of Nursing, School of Medicine, Tokai University, 143 Shimokasuya, Isehara 259-1193, Kanagawa, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Hee-Jae Cha
Int. J. Mol. Sci. 2021, 22(9), 4504; https://doi.org/10.3390/ijms22094504
Received: 28 February 2021 / Revised: 23 April 2021 / Accepted: 23 April 2021 / Published: 26 April 2021
(This article belongs to the Special Issue Endogenous Retroviruses: Functions at Molecular Level)
(1) Background: The ERVPb1 gene in humans is derived from an envelope (Env) gene of a human endogenous retrovirus group, HERV-P(b). The ERVPb1 gene reportedly has a conserved open reading frame (ORF) in Old World monkeys. Although its forced expression led to cell-fusion in an ex vivo cell culture system, like other Env-derived genes such as syncytin-1 and -2, its mRNA expression is not placenta-specific, but almost ubiquitous, albeit being quite low in human tissues and organs, implying a distinct role for ERVPb1. (2) Methods: To elucidate the cell lineage(s) in which the ERVPb1 protein is translated in human development, we developed a novel, highly sensitive system for detecting HERV-derived proteins/peptides expressed in the tissue differentiation process of human induced pluripotent stem cells (iPSCs). (3) Results: We first determined that ERVPb1 is also conserved in New World monkeys. Then, we showed that the ERVPb1 protein is translated from a uniquely spliced ERVPb1 transcript in hematopoietic cell lineages, including a subset of macrophages, and further showed that its mRNA expression is upregulated by lipopolysaccharide (LPS) stimulation in primary human monocytes. (4) Conclusions: ERVPb1 is unique to Simiiformes and actually translated in hematopoietic cell lineages, including a subset of macrophages. View Full-Text
Keywords: an Env-derived ERVPb1 gene; a simiiforme-specific gene; retrovirus-derived genes (RVDs); human genome; hemangioblast cells; hematopoietic cells; macrophages; human iPSCs; organoid generation an Env-derived ERVPb1 gene; a simiiforme-specific gene; retrovirus-derived genes (RVDs); human genome; hemangioblast cells; hematopoietic cells; macrophages; human iPSCs; organoid generation
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MDPI and ACS Style

Matsuzawa, A.; Lee, J.; Nakagawa, S.; Itoh, J.; Takahashi Ueda, M.; Mitsuhashi, S.; Kochi, Y.; Kaneko-Ishino, T.; Ishino, F. HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages. Int. J. Mol. Sci. 2021, 22, 4504. https://doi.org/10.3390/ijms22094504

AMA Style

Matsuzawa A, Lee J, Nakagawa S, Itoh J, Takahashi Ueda M, Mitsuhashi S, Kochi Y, Kaneko-Ishino T, Ishino F. HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages. International Journal of Molecular Sciences. 2021; 22(9):4504. https://doi.org/10.3390/ijms22094504

Chicago/Turabian Style

Matsuzawa, Ayumi, Jiyoung Lee, So Nakagawa, Johbu Itoh, Mahoko Takahashi Ueda, Satomi Mitsuhashi, Yuta Kochi, Tomoko Kaneko-Ishino, and Fumitoshi Ishino. 2021. "HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages" International Journal of Molecular Sciences 22, no. 9: 4504. https://doi.org/10.3390/ijms22094504

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