HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages
Abstract
:1. Introduction
2. Results
2.1. ERVPb1 Is Unique to Simiiformes and Exhibits a Quite Low Level of Expression in Limited Tissues and Organs in Humans
2.2. Generation of Human iPSCs with an ERVPb1–Venus Fusion Gene in Its Endogenous Locus
2.3. Detection of the ERVPb1–Venus Fusion Protein in a Hematopoietic Cell Lineage
3. Discussion
4. Materials and Methods
4.1. Comparison of ERVPb1 ORF in Simiiformes
4.2. Analysis of Short Read RNA-Seq Data
4.3. Generation of Human iPSCs with an ERVPb1-NV Construct
4.4. Culture and Differentiation of Human iPSCs
4.5. Gene Expression Analysis by Real-Time PCR
4.6. Rapid Amplification of cDNA Ends (RACE)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Matsuzawa, A.; Lee, J.; Nakagawa, S.; Itoh, J.; Takahashi Ueda, M.; Mitsuhashi, S.; Kochi, Y.; Kaneko-Ishino, T.; Ishino, F. HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages. Int. J. Mol. Sci. 2021, 22, 4504. https://doi.org/10.3390/ijms22094504
Matsuzawa A, Lee J, Nakagawa S, Itoh J, Takahashi Ueda M, Mitsuhashi S, Kochi Y, Kaneko-Ishino T, Ishino F. HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages. International Journal of Molecular Sciences. 2021; 22(9):4504. https://doi.org/10.3390/ijms22094504
Chicago/Turabian StyleMatsuzawa, Ayumi, Jiyoung Lee, So Nakagawa, Johbu Itoh, Mahoko Takahashi Ueda, Satomi Mitsuhashi, Yuta Kochi, Tomoko Kaneko-Ishino, and Fumitoshi Ishino. 2021. "HERV-Derived Ervpb1 Is Conserved in Simiiformes, Exhibiting Expression in Hematopoietic Cell Lineages Including Macrophages" International Journal of Molecular Sciences 22, no. 9: 4504. https://doi.org/10.3390/ijms22094504