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Article

Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

1
Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
2
Lucideon Ltd., Penkhull, Stoke-on-Trent, Staffordshire ST4 7LQ, UK
3
CAM Bioceramics B.V., 2333 CL Leiden, The Netherlands
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Department of Materials Science and Engineering, Institute of Space Technology Islamabad, Islamabad 44000, Pakistan
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GeoZentrum Nordbayern, Mineralogy, University of Erlangen-Nuremberg, 91054 Erlangen, Germany
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Department of Biophysics, University of Erlangen-Nuremberg, 91052 Erlangen, Germany
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Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad Lahore Campus, Defence Road, Off-Raiwind Road, Lahore 54000, Pakistan
*
Author to whom correspondence should be addressed.
Academic Editor: Young-Jin Kim
Int. J. Mol. Sci. 2021, 22(8), 4246; https://doi.org/10.3390/ijms22084246
Received: 10 March 2021 / Revised: 6 April 2021 / Accepted: 12 April 2021 / Published: 19 April 2021
(This article belongs to the Special Issue Bone Substitute Material)
In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications. View Full-Text
Keywords: co-substituted hydroxyapatite; strontium; selenium; antibacterial; cytocompatibility co-substituted hydroxyapatite; strontium; selenium; antibacterial; cytocompatibility
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MDPI and ACS Style

Maqbool, M.; Nawaz, Q.; Atiq Ur Rehman, M.; Cresswell, M.; Jackson, P.; Hurle, K.; Detsch, R.; Goldmann, W.H.; Shah, A.T.; Boccaccini, A.R. Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite. Int. J. Mol. Sci. 2021, 22, 4246. https://doi.org/10.3390/ijms22084246

AMA Style

Maqbool M, Nawaz Q, Atiq Ur Rehman M, Cresswell M, Jackson P, Hurle K, Detsch R, Goldmann WH, Shah AT, Boccaccini AR. Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite. International Journal of Molecular Sciences. 2021; 22(8):4246. https://doi.org/10.3390/ijms22084246

Chicago/Turabian Style

Maqbool, Muhammad, Qaisar Nawaz, Muhammad Atiq Ur Rehman, Mark Cresswell, Phil Jackson, Katrin Hurle, Rainer Detsch, Wolfgang H. Goldmann, Asma T. Shah, and Aldo R. Boccaccini 2021. "Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite" International Journal of Molecular Sciences 22, no. 8: 4246. https://doi.org/10.3390/ijms22084246

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