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Open AccessArticle

Crystal Structure of the Epo1-Bem3 Complex for Bud Growth

by 1,2,†, 1,†, 3, 4 and 2,*
1
State Key Laboratory of Biotherapy, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China
2
Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Medicine, Tsinghua University, Beijing 100084, China
3
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530000, China
4
iHuman Institute, ShanghaiTech University, Shanghai 201210, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Shin-ichi Tate
Int. J. Mol. Sci. 2021, 22(8), 3812; https://doi.org/10.3390/ijms22083812
Received: 15 March 2021 / Revised: 30 March 2021 / Accepted: 4 April 2021 / Published: 7 April 2021
(This article belongs to the Special Issue Protein Structure Dynamics and Function)
Tubules of the endoplasmic reticulum (ER) spread into the buds of yeast by an actin-based mechanism and, upon entry, become attached to the polarisome, a proteinaceous micro-compartment below the tip of the bud. The minimal tether between polarisome and cortical ER is formed by a protein complex consisting of Epo1, a member of the polarisome, Scs2, a membrane protein of the ER and Cdc42 guanosine triphosphatase-activating protein Bem3. Here, we report the crystal structure of a complex between Epo1 and Bem3. In addition, we characterize through the hydrogen/deuterium (H/D) exchange assay the interface between Scs2 and Epo1. Our findings provide a first structural insight into the molecular architecture of the link between cortical ER and the polarisome. View Full-Text
Keywords: budding yeast; polarisome; Epo1-Bem3; Ssc2; complex budding yeast; polarisome; Epo1-Bem3; Ssc2; complex
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MDPI and ACS Style

Wang, J.; Li, L.; Ming, Z.; Wu, L.; Yan, L. Crystal Structure of the Epo1-Bem3 Complex for Bud Growth. Int. J. Mol. Sci. 2021, 22, 3812. https://doi.org/10.3390/ijms22083812

AMA Style

Wang J, Li L, Ming Z, Wu L, Yan L. Crystal Structure of the Epo1-Bem3 Complex for Bud Growth. International Journal of Molecular Sciences. 2021; 22(8):3812. https://doi.org/10.3390/ijms22083812

Chicago/Turabian Style

Wang, Jin; Li, Lei; Ming, Zhenhua; Wu, Lijie; Yan, Liming. 2021. "Crystal Structure of the Epo1-Bem3 Complex for Bud Growth" Int. J. Mol. Sci. 22, no. 8: 3812. https://doi.org/10.3390/ijms22083812

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