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Open AccessArticle

Crystal Structure of the Epo1-Bem3 Complex for Bud Growth

by 1,2,†, 1,†, 3, 4 and 2,*
State Key Laboratory of Biotherapy, West China Hospital, Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, China
Laboratory of Structural Biology and MOE Laboratory of Protein Science, School of Medicine, Tsinghua University, Beijing 100084, China
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530000, China
iHuman Institute, ShanghaiTech University, Shanghai 201210, China
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Shin-ichi Tate
Int. J. Mol. Sci. 2021, 22(8), 3812;
Received: 15 March 2021 / Revised: 30 March 2021 / Accepted: 4 April 2021 / Published: 7 April 2021
(This article belongs to the Special Issue Protein Structure Dynamics and Function)
Tubules of the endoplasmic reticulum (ER) spread into the buds of yeast by an actin-based mechanism and, upon entry, become attached to the polarisome, a proteinaceous micro-compartment below the tip of the bud. The minimal tether between polarisome and cortical ER is formed by a protein complex consisting of Epo1, a member of the polarisome, Scs2, a membrane protein of the ER and Cdc42 guanosine triphosphatase-activating protein Bem3. Here, we report the crystal structure of a complex between Epo1 and Bem3. In addition, we characterize through the hydrogen/deuterium (H/D) exchange assay the interface between Scs2 and Epo1. Our findings provide a first structural insight into the molecular architecture of the link between cortical ER and the polarisome. View Full-Text
Keywords: budding yeast; polarisome; Epo1-Bem3; Ssc2; complex budding yeast; polarisome; Epo1-Bem3; Ssc2; complex
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MDPI and ACS Style

Wang, J.; Li, L.; Ming, Z.; Wu, L.; Yan, L. Crystal Structure of the Epo1-Bem3 Complex for Bud Growth. Int. J. Mol. Sci. 2021, 22, 3812.

AMA Style

Wang J, Li L, Ming Z, Wu L, Yan L. Crystal Structure of the Epo1-Bem3 Complex for Bud Growth. International Journal of Molecular Sciences. 2021; 22(8):3812.

Chicago/Turabian Style

Wang, Jin; Li, Lei; Ming, Zhenhua; Wu, Lijie; Yan, Liming. 2021. "Crystal Structure of the Epo1-Bem3 Complex for Bud Growth" Int. J. Mol. Sci. 22, no. 8: 3812.

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